Human mitochondrial mRNAs are stabilized with polyadenylation regulated by mitochondria-specific poly(A) polymerase and polynucleotide phosphorylase

Takashi Nagaike, Tsutomu Suzuki, Takayuki Katoh, Takuya Ueda

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Mammalian mitochondrial (mt) mRNAs have short poly(A) tails at their 3′ termini that are post-transcriptionally synthesized by mt poly(A) polymerase (PAP). The polyadenylation of mt mRNAs is known to be a key process needed to create UAA stop codons that are not encoded in mtDNA. In some cases, polyadenylation is required for the tRNA maturation by editing of its 3′ terminus. However, little is known about the functional roles the poly(A) tail of mt mRNAs plays in mt translation and RNA turnover. Here we show human mt PAP (hmtPAP) and human polynucleotide phosphorylase (hPNPase) control poly(A) synthesis in human mitochondria. Partial inactivation of hmtPAP by RNA interference using small interfering RNA in HeLa cells resulted in shortened poly(A) tails and decreased steady state levels of some mt mRNAs as well as their translational products. Moreover, knocking down hmtPAP generated markedly defective mt membrane potentials and reduced oxygen consumption. In contrast, knocking down hPNPase showed significantly extended poly(A) tails of mt mRNAs. These results demonstrate that the poly(A) length of human mt mRNAs is controlled by polyadenylation by hmtPAP and deadenylation by hPNPase, and polyadenylation is required for the stability of mt mRNAs.

Original languageEnglish
Pages (from-to)19721-19727
Number of pages7
JournalJournal of Biological Chemistry
Volume280
Issue number20
DOIs
Publication statusPublished - 2005 May 20
Externally publishedYes

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Polyribonucleotide Nucleotidyltransferase
Polynucleotide Adenylyltransferase
Polyadenylation
Mitochondria
Messenger RNA
Poly A
RNA
Transfer RNA
mitochondrial messenger RNA
Mitochondrial DNA
Small Interfering RNA
Terminator Codon
Mitochondrial Membrane Potential
RNA Interference
HeLa Cells
Oxygen Consumption
Oxygen
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Human mitochondrial mRNAs are stabilized with polyadenylation regulated by mitochondria-specific poly(A) polymerase and polynucleotide phosphorylase. / Nagaike, Takashi; Suzuki, Tsutomu; Katoh, Takayuki; Ueda, Takuya.

In: Journal of Biological Chemistry, Vol. 280, No. 20, 20.05.2005, p. 19721-19727.

Research output: Contribution to journalArticle

Nagaike, Takashi ; Suzuki, Tsutomu ; Katoh, Takayuki ; Ueda, Takuya. / Human mitochondrial mRNAs are stabilized with polyadenylation regulated by mitochondria-specific poly(A) polymerase and polynucleotide phosphorylase. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 20. pp. 19721-19727.
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