Human origin recognition complex binds preferentially to G-quadruplex-preferable RNA and single-stranded DNA

Shoko Hoshina, Kei Yura, Honami Teranishi, Noriko Kiyasu, Ayumi Tominaga, Haruka Kadoma, Ayaka Nakatsuka, Tomoko Kunichika, Chikashi Obuse, Shou Waga

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Background: ORC binds to replication origins, but human ORC does not exhibit apparent sequence-specificity. Results: G-quadruplex (G4)-preferable RNA or single-stranded DNA competes for DNA binding of ORC. Conclusion: HumanORCbinds preferentially toRNAand single-strandedDNAthat form G4, and the certain domain in ORC1 is involved in this binding. Significance: This ability may correlate with the G4-formable motif in human replication origins.

Original languageEnglish
Pages (from-to)30161-30171
Number of pages11
JournalJournal of Biological Chemistry
Volume288
Issue number42
DOIs
Publication statusPublished - 2013 Oct 18
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Hoshina, S., Yura, K., Teranishi, H., Kiyasu, N., Tominaga, A., Kadoma, H., Nakatsuka, A., Kunichika, T., Obuse, C., & Waga, S. (2013). Human origin recognition complex binds preferentially to G-quadruplex-preferable RNA and single-stranded DNA. Journal of Biological Chemistry, 288(42), 30161-30171. https://doi.org/10.1074/jbc.M113.492504