Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing

Yuichi Morozumi, Ryohei Ino, Motoki Takaku, Mihoko Hosokawa, Shinichiro Chuma, Hitoshi Kurumizaka

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    PSF is considered to have multiple functions in RNA processing, transcription and DNA repair by mitotic recombination. In the present study, we found that PSF is produced in spermatogonia, spermatocytes and spermatids, suggesting that PSF may also function in meiotic recombination. We tested the effect of PSF on homologous pairing by the meiosis-specific recombinase DMC1, and found that human PSF robustly stimulated it. PSF synergistically enhanced the formation of a synaptic complex containing DMC1, ssDNA and dsDNA during homologous pairing. The PSF-mediated DMC1 stimulation may be promoted by its DNA aggregation activity, which increases the local concentrations of ssDNA and dsDNA for homologous pairing by DMC1. These results suggested that PSF may function as an activator for the meiosis-specific recombinase DMC1 in higher eukaryotes.

    Original languageEnglish
    Pages (from-to)3031-3041
    Number of pages11
    JournalNucleic Acids Research
    Volume40
    Issue number7
    DOIs
    Publication statusPublished - 2012 Apr

    Fingerprint

    Meiosis
    Genetic Recombination
    Activator Appliances
    Spermatogonia
    Recombinases
    Spermatocytes
    Spermatids
    DNA
    Eukaryota
    DNA Repair
    RNA
    human DMC1 protein

    ASJC Scopus subject areas

    • Genetics

    Cite this

    Morozumi, Y., Ino, R., Takaku, M., Hosokawa, M., Chuma, S., & Kurumizaka, H. (2012). Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing. Nucleic Acids Research, 40(7), 3031-3041. https://doi.org/10.1093/nar/gkr1229

    Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing. / Morozumi, Yuichi; Ino, Ryohei; Takaku, Motoki; Hosokawa, Mihoko; Chuma, Shinichiro; Kurumizaka, Hitoshi.

    In: Nucleic Acids Research, Vol. 40, No. 7, 04.2012, p. 3031-3041.

    Research output: Contribution to journalArticle

    Morozumi, Y, Ino, R, Takaku, M, Hosokawa, M, Chuma, S & Kurumizaka, H 2012, 'Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing', Nucleic Acids Research, vol. 40, no. 7, pp. 3031-3041. https://doi.org/10.1093/nar/gkr1229
    Morozumi, Yuichi ; Ino, Ryohei ; Takaku, Motoki ; Hosokawa, Mihoko ; Chuma, Shinichiro ; Kurumizaka, Hitoshi. / Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing. In: Nucleic Acids Research. 2012 ; Vol. 40, No. 7. pp. 3031-3041.
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