Human tyrosine tRNA is also internally cleavable by E. coli ribonuclease P RNA ribozyme in vitro

Tomoaki Ando, Terumichi Tanaka, Yoshiaki Hori, Etsuko Sakai, Yo Kikuchi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Transfer RNA is an essential molecule for biological system, and each (RNA molecule commonly has a cloverleaf structure. Previously, we experimentally showed that some Drosophila tRNA (tRNAAla, tRNAHis, and tRNAMet 1) molecules fit to form another, non-cloverleaf, structure in which the 3′-half of the tRNA molecules forms an alternative hairpin, and that the tRNA molecules are internally cleaved by the catalytic RNA of bacterial ribonuclease P (RNase P). Until now, the hyperprocessing reaction of tRNA has only been reported with Drosophila tRNAs. This time, we applied the hyperprocessing reaction to one of human tRNAs, human tyrosine tRNA, and we showed that this tRNA was also hyperprocessed by E. coli RNase P RNA. This tRNA is the first example for hyperprocessed non-Drosophila tRNAs. The results suggest that the hyperprocessing reaction can be a useful tool to detect destablized tRNA molecules from any species.

Original languageEnglish
Pages (from-to)2798-2801
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume65
Issue number12
DOIs
Publication statusPublished - 2001 Dec
Externally publishedYes

Fingerprint

Catalytic RNA
ribonucleases
Transfer RNA
RNA
Escherichia coli
Tyrosine
tyrosine
Drosophila
transfer RNA
Molecules
E coli ribonuclease P
In Vitro Techniques
Ribonucleases
RNA, Transfer, His
RNA, Transfer, Ala
Ribonuclease P
Biological systems

Keywords

  • Escherichia coli
  • Hyperprocessing
  • RNase P
  • tRNA
  • Tyrosine

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Human tyrosine tRNA is also internally cleavable by E. coli ribonuclease P RNA ribozyme in vitro. / Ando, Tomoaki; Tanaka, Terumichi; Hori, Yoshiaki; Sakai, Etsuko; Kikuchi, Yo.

In: Bioscience, Biotechnology and Biochemistry, Vol. 65, No. 12, 12.2001, p. 2798-2801.

Research output: Contribution to journalArticle

Ando, Tomoaki ; Tanaka, Terumichi ; Hori, Yoshiaki ; Sakai, Etsuko ; Kikuchi, Yo. / Human tyrosine tRNA is also internally cleavable by E. coli ribonuclease P RNA ribozyme in vitro. In: Bioscience, Biotechnology and Biochemistry. 2001 ; Vol. 65, No. 12. pp. 2798-2801.
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