Hybridization of deoxyribonucleic acid and immobilization of green fluorescent protein on nanostructured organosilane templates

Takashi Tanii, Takumi Hosaka, Takeo Miyake, Yuzo Kanari, Guo Jun Zhang, Takashi Funatsu, Iwao Ohdomari

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We propose a novel process for preferential immobilization of deoxyribonucleic acid (DNA) and green fluorescent protein (GFP) onto organosilane self-assembled monolayer (SAM) templates. One of the advantages of using the organosilane SAM as the template is that it is electron-beam sensitive and, by utilizing the SAM as an alternative resist film, we can make nanopatterns on a molecular scale because the proximity effect is negligible owing to the film's thinness. An other advantage is that the organosilane SAM is repellent to both DNA and GFP. Thus, the patterned SAM can be utilized as the passivation film covering the outside of the pattern while DNA and GFP are immobilized within the pattern. We investigate two kinds of organosilane SAMs for the template. One is n-octadecyltrimethoxysilane (ODS) SAM, and the other is 1H,1H,2H,2H-perfluorodecyltriethoxysilane (FDS) SAM. Our results indicate that the ODS SAM is more repellent to DNA than the FDS SAM and is suitable for DNA immobilization, while the FDS SAM template is suitable for GFP immobilization via biotinavidin coupling because the FDS SAM surface prevents the nonspecific adsorption of streptavidin. Although the nonspecific adsorption of DNA and GFP on a SAM is also recognized, by controlling both the concentration and the incubation time, we can immobilize DNA and GFP preferentially onto nanopatterns of 100 nm diameter.

Original languageEnglish
Pages (from-to)5851-5855
Number of pages5
JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume44
Issue number7 B
DOIs
Publication statusPublished - 2005 Jul 26

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Keywords

  • Deoxyribonucleic acid
  • DNA
  • Electron-beam lithography
  • GFP
  • Green fluorescent protein
  • Organosilane
  • SAM
  • Self-assembled monolayer

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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