Hydrodynamic Evaluation of Three-Dimensional Adsorption of Protein to a Polymer Chain Grafted onto a Porous Substrate

Satoshi Tsuneda, HIROKI KAGAWA, KYOICHI SAITO, TAKANOBU SUGO

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27 Citations (Scopus)

Abstract

A porous hollow-fiber membrane was modified with diethylamino (DEA) and ethanolamino (EA) groups by radiation-induced graft polymerization and subsequent chemical modifications. Adsorption behavior of bovine serum albumin (BSA) was investigated by permeating a BSA buffer solution through the membrane. A polymer chain grafted onto the pore surface of the membrane held BSA in multilayers, i.e., three-dimensionally in a tentacle-like manner. The membrane with a DEA group density of 2.5 mol/kg had a binding capacity for BSA of 443 g/kg, equivalent to 11 times the monolayer adsorption. Permeation of a buffer solution containing 0.5MNaCl as an eluent caused the graft chain to shrink, resulting in restricted diffusion of BSA departing from the graft chain toward the pore interior while realizing a quantitative elution. Hydrodynamic analysis of the change in permeation pressure accompanying BSA binding to the graft chain showed that the BSA molecules layered in the graft chains form a closely packed structure.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalJournal of Colloid and Interface Science
Volume176
Issue number1
DOIs
Publication statusPublished - 1995 Dec 1
Externally publishedYes

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Keywords

  • graft chain
  • hydrodynamic evaluation
  • porous membrane
  • protein adsorption
  • three-dimensional adsorption

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Colloid and Surface Chemistry

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