Hydrogen evolution by co-immobilized Chlorella vulgaris and Clostridium butyricum cells

Hiromichi Kayano, Tadashi Matsunaga, Isao Karube, Shuichi Suzuki

Research output: Contribution to journalArticle

21 Citations (Scopus)


Whole cells of Chlorella vulgaris and Clostridium butyricum were co-immobilized in 2% agar gel. NADP was suitable as an electron carrier. The rate of hydrogen evolution increased with increasing NADP concentration. The optimum conditions for hydrogen evolution were pH 7.0 and 37°C. The immobilized C. vulgaris-NADP-immobilized Cl. butyricum system continuously evolved hydrogen at a rate of 0.29-1.34 μmol/h per mg Chl for 6 days. On the other hand, the system without NADP evolved only a trace amount of hydrogen.

Original languageEnglish
Pages (from-to)80-85
Number of pages6
JournalBBA - Bioenergetics
Issue number1
Publication statusPublished - 1981 Nov 12
Externally publishedYes



  • (Chlorella vulgaris, Cl. butyricum)
  • Hydrogen evolution
  • Immobilized cell
  • Water photolysis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this