Hydrogen evolution by co-immobilized chloroplasts and Clostridium butyricum

Isao Karube, Tadashi Matsunaga, Toshiaki Otsuka, Hiromichi Kayano, Shuichi Suzuki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Spinach chloroplasts and Clostridium butyricum cells were immobilized in 2% agar gel. Crude ferredoxin isolated from spinach and benzyl viologen were used as electron carriers. The optimum pH for both NADP reduction by immobilized chloroplasts and for hydrogen evolution by immobilized Cl. butyricum was 8.0. The optimum temperature was between 25 and 30°C for NADP reduction by immobilized chloroplasts, and 37°C for hydrogen evolution by immobilized cells. The total amount of hydrogen evolved in 6 h was 41 μmol/mg Chl for the immobilized chloroplast-benzyl viologen-immobilized Cl. butyricum system, and 11 μmol/mg Chl for the immobilized chloroplast-ferredoxin-Cl. butyricum system. The systems evolved only a trace amount of hydrogen when dichlorophenyldimethylurea was added. The immobilized chloroplast-benzyl viologen-immobilized Cl. butyricum system evolved hydrogen continuously for 6 h, and immobilized Cl. butyricum retained the initial hydrogenase activity. However, the photoreduction activity of chloroplasts decreased to 30% of the initial activity after 6 h of reaction.

Original languageEnglish
Pages (from-to)490-495
Number of pages6
JournalBBA - Bioenergetics
Volume637
Issue number3
DOIs
Publication statusPublished - 1981 Oct 12
Externally publishedYes

Keywords

  • (Cl. butyricum, Spinach)
  • Hydrogen evolution
  • Hydrogenase
  • Immobilized bacteria
  • Immobilized chloroplast
  • Water photolysis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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  • Cite this

    Karube, I., Matsunaga, T., Otsuka, T., Kayano, H., & Suzuki, S. (1981). Hydrogen evolution by co-immobilized chloroplasts and Clostridium butyricum. BBA - Bioenergetics, 637(3), 490-495. https://doi.org/10.1016/0005-2728(81)90055-4