TY - JOUR
T1 - Hydrogen water intake may suppress liver glycogen utilization without affecting redox biomarkers during exercise in rats
AU - Kawamura, Takuji
AU - Fujii, Ryosuke
AU - Higashida, Kazuhiko
AU - Muraoka, Isao
N1 - Funding Information:
This research was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI (grant no. 15K12674).*%blankline%*
Publisher Copyright:
© 2018 Edizioni Minerva Medica.
PY - 2019
Y1 - 2019
N2 - Background: Studies have revealed that molecular hydrogen (H2) exhibits therapeutic effects against various diseases through biological activities such as antioxidation. However, little is known about the effects of H2 on redox biomarkers and related indicators during exercise. Therefore, we examined whether 2 weeks of H2 water intake could affect the redox biomarkers, energy metabolism, and performance level of rats during endurance exercise. Methods: Male Sprague-Dawley rats (N.=32) were randomly divided into four groups: control (C), control-exercise (C-Ex), H2 water (H2), and H2 water-exercise (H2-Ex). Each group was given mineral water (C and C-Ex) or H2 water (H2 and H2-Ex) ad libitum for 2 weeks. Then the C-Ex and H2-Ex groups were subjected to an exercise performance test. Blood, skeletal muscle, and liver samples were analysed for redox biomarkers, blood energy substrates, and tissue glycogen contents. Results: Two-way ANOVA revealed that H2 water intake did not affect redox biomarkers (thiobarbituric acid-reactive substances, protein carbonyl, and total antioxidant capacity), blood energy substrates (lactate, glucose, free fatty acid, and triglyceride), and muscle glycogen content. However, liver glycogen content was significantly higher in the H2-Ex group than in the C-Ex group (P < 0.01). Conclusions: The results suggest that 2 weeks of H2 water intake did not affect redox biomarkers, blood energy substrates, muscle glycogen content, and performance level during endurance exercise: however, it may slightly suppress liver glycogen utilization during exercise.
AB - Background: Studies have revealed that molecular hydrogen (H2) exhibits therapeutic effects against various diseases through biological activities such as antioxidation. However, little is known about the effects of H2 on redox biomarkers and related indicators during exercise. Therefore, we examined whether 2 weeks of H2 water intake could affect the redox biomarkers, energy metabolism, and performance level of rats during endurance exercise. Methods: Male Sprague-Dawley rats (N.=32) were randomly divided into four groups: control (C), control-exercise (C-Ex), H2 water (H2), and H2 water-exercise (H2-Ex). Each group was given mineral water (C and C-Ex) or H2 water (H2 and H2-Ex) ad libitum for 2 weeks. Then the C-Ex and H2-Ex groups were subjected to an exercise performance test. Blood, skeletal muscle, and liver samples were analysed for redox biomarkers, blood energy substrates, and tissue glycogen contents. Results: Two-way ANOVA revealed that H2 water intake did not affect redox biomarkers (thiobarbituric acid-reactive substances, protein carbonyl, and total antioxidant capacity), blood energy substrates (lactate, glucose, free fatty acid, and triglyceride), and muscle glycogen content. However, liver glycogen content was significantly higher in the H2-Ex group than in the C-Ex group (P < 0.01). Conclusions: The results suggest that 2 weeks of H2 water intake did not affect redox biomarkers, blood energy substrates, muscle glycogen content, and performance level during endurance exercise: however, it may slightly suppress liver glycogen utilization during exercise.
KW - Athletic performance
KW - Biomarkers
KW - Glycogen
KW - Oxidation-reduction
KW - Physical endurance
KW - Rats
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U2 - 10.23736/S0393-3660.18.03912-8
DO - 10.23736/S0393-3660.18.03912-8
M3 - Article
AN - SCOPUS:85075698561
SN - 0393-3660
VL - 178
SP - 611
EP - 617
JO - Gazzetta Medica Italiana Archivio per le Scienze Mediche
JF - Gazzetta Medica Italiana Archivio per le Scienze Mediche
IS - 9
ER -