Abstract
For preparing a thermally modulated biointerface that separates cells without the modification of cell surfaces for regenerative medicine and tissue engineering, poly(N-isopropylacrylamide-co-butyl methacrylate) (P(IPAAm-co-BMA), thermo-responsive hydrophobic copolymer brushes with various BMA composition were formed on glass substrate through a surface-initiated atom transfer radical polymerization (ATRP). Characterization of the prepared surface was performed by X-ray photoelectron spectroscopy (XPS), attenuated total reflection Fourier transform infrared spectroscopy (ATR/FT-IR), and gel-permeation chromatography (GPC) measurement. Prepared copolymer brush surfaces were characterized by observing the adhesion (37 C) and detachment (20 or 10 C) of four types of human cells: human umbilical vein endothelial cells (HUVECs), normal human dermal fibroblasts (NHDFs), human aortic smooth muscle cells (SMCs), and human skeletal muscle myoblast cells (HSMMs). HUVECs and NHDFs exhibited their effective detachment temperature at 20 and 10 C, respectively. Using cells' intrinsic temperature sensitivity for detachment from the copolymer brush, a mixture of green fluorescent protein (GFP)-expressing HUVECs (GFP-HUVECs) and NHDFs was separated.
| Original language | English |
|---|---|
| Pages (from-to) | 3423-3433 |
| Number of pages | 11 |
| Journal | Biomacromolecules |
| Volume | 14 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2013 Oct 14 |
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ASJC Scopus subject areas
- Bioengineering
- Materials Chemistry
- Polymers and Plastics
- Biomaterials
- Medicine(all)
Cite this
Hydrophobized thermoresponsive copolymer brushes for cell separation by multistep temperature change. / Nagase, Kenichi; Hatakeyama, Yuri; Shimizu, Tatsuya; Matsuura, Katsuhisa; Yamato, Masayuki; Takeda, Naoya; Okano, Teruo.
In: Biomacromolecules, Vol. 14, No. 10, 14.10.2013, p. 3423-3433.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Hydrophobized thermoresponsive copolymer brushes for cell separation by multistep temperature change
AU - Nagase, Kenichi
AU - Hatakeyama, Yuri
AU - Shimizu, Tatsuya
AU - Matsuura, Katsuhisa
AU - Yamato, Masayuki
AU - Takeda, Naoya
AU - Okano, Teruo
PY - 2013/10/14
Y1 - 2013/10/14
N2 - For preparing a thermally modulated biointerface that separates cells without the modification of cell surfaces for regenerative medicine and tissue engineering, poly(N-isopropylacrylamide-co-butyl methacrylate) (P(IPAAm-co-BMA), thermo-responsive hydrophobic copolymer brushes with various BMA composition were formed on glass substrate through a surface-initiated atom transfer radical polymerization (ATRP). Characterization of the prepared surface was performed by X-ray photoelectron spectroscopy (XPS), attenuated total reflection Fourier transform infrared spectroscopy (ATR/FT-IR), and gel-permeation chromatography (GPC) measurement. Prepared copolymer brush surfaces were characterized by observing the adhesion (37 C) and detachment (20 or 10 C) of four types of human cells: human umbilical vein endothelial cells (HUVECs), normal human dermal fibroblasts (NHDFs), human aortic smooth muscle cells (SMCs), and human skeletal muscle myoblast cells (HSMMs). HUVECs and NHDFs exhibited their effective detachment temperature at 20 and 10 C, respectively. Using cells' intrinsic temperature sensitivity for detachment from the copolymer brush, a mixture of green fluorescent protein (GFP)-expressing HUVECs (GFP-HUVECs) and NHDFs was separated.
AB - For preparing a thermally modulated biointerface that separates cells without the modification of cell surfaces for regenerative medicine and tissue engineering, poly(N-isopropylacrylamide-co-butyl methacrylate) (P(IPAAm-co-BMA), thermo-responsive hydrophobic copolymer brushes with various BMA composition were formed on glass substrate through a surface-initiated atom transfer radical polymerization (ATRP). Characterization of the prepared surface was performed by X-ray photoelectron spectroscopy (XPS), attenuated total reflection Fourier transform infrared spectroscopy (ATR/FT-IR), and gel-permeation chromatography (GPC) measurement. Prepared copolymer brush surfaces were characterized by observing the adhesion (37 C) and detachment (20 or 10 C) of four types of human cells: human umbilical vein endothelial cells (HUVECs), normal human dermal fibroblasts (NHDFs), human aortic smooth muscle cells (SMCs), and human skeletal muscle myoblast cells (HSMMs). HUVECs and NHDFs exhibited their effective detachment temperature at 20 and 10 C, respectively. Using cells' intrinsic temperature sensitivity for detachment from the copolymer brush, a mixture of green fluorescent protein (GFP)-expressing HUVECs (GFP-HUVECs) and NHDFs was separated.
UR - http://www.scopus.com/inward/record.url?scp=84885655542&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885655542&partnerID=8YFLogxK
U2 - 10.1021/bm4006722
DO - 10.1021/bm4006722
M3 - Article
C2 - 24003766
AN - SCOPUS:84885655542
VL - 14
SP - 3423
EP - 3433
JO - Biomacromolecules
JF - Biomacromolecules
SN - 1525-7797
IS - 10
ER -