Hydrophobized thermoresponsive copolymer brushes for cell separation by multistep temperature change

Kenichi Nagase, Yuri Hatakeyama, Tatsuya Shimizu, Katsuhisa Matsuura, Masayuki Yamato, Naoya Takeda, Teruo Okano

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    For preparing a thermally modulated biointerface that separates cells without the modification of cell surfaces for regenerative medicine and tissue engineering, poly(N-isopropylacrylamide-co-butyl methacrylate) (P(IPAAm-co-BMA), thermo-responsive hydrophobic copolymer brushes with various BMA composition were formed on glass substrate through a surface-initiated atom transfer radical polymerization (ATRP). Characterization of the prepared surface was performed by X-ray photoelectron spectroscopy (XPS), attenuated total reflection Fourier transform infrared spectroscopy (ATR/FT-IR), and gel-permeation chromatography (GPC) measurement. Prepared copolymer brush surfaces were characterized by observing the adhesion (37 C) and detachment (20 or 10 C) of four types of human cells: human umbilical vein endothelial cells (HUVECs), normal human dermal fibroblasts (NHDFs), human aortic smooth muscle cells (SMCs), and human skeletal muscle myoblast cells (HSMMs). HUVECs and NHDFs exhibited their effective detachment temperature at 20 and 10 C, respectively. Using cells' intrinsic temperature sensitivity for detachment from the copolymer brush, a mixture of green fluorescent protein (GFP)-expressing HUVECs (GFP-HUVECs) and NHDFs was separated.

    Original languageEnglish
    Pages (from-to)3423-3433
    Number of pages11
    JournalBiomacromolecules
    Volume14
    Issue number10
    DOIs
    Publication statusPublished - 2013 Oct 14

    Fingerprint

    Cell Separation
    Brushes
    Endothelial cells
    Fibroblasts
    Copolymers
    Temperature
    Human Umbilical Vein Endothelial Cells
    Muscle
    Cells
    Skin
    Atom transfer radical polymerization
    Gel permeation chromatography
    Green Fluorescent Proteins
    Tissue engineering
    Skeletal Myoblasts
    Fourier transform infrared spectroscopy
    Photoelectron Spectroscopy
    Regenerative Medicine
    Adhesion
    X ray photoelectron spectroscopy

    ASJC Scopus subject areas

    • Bioengineering
    • Materials Chemistry
    • Polymers and Plastics
    • Biomaterials
    • Medicine(all)

    Cite this

    Nagase, K., Hatakeyama, Y., Shimizu, T., Matsuura, K., Yamato, M., Takeda, N., & Okano, T. (2013). Hydrophobized thermoresponsive copolymer brushes for cell separation by multistep temperature change. Biomacromolecules, 14(10), 3423-3433. https://doi.org/10.1021/bm4006722

    Hydrophobized thermoresponsive copolymer brushes for cell separation by multistep temperature change. / Nagase, Kenichi; Hatakeyama, Yuri; Shimizu, Tatsuya; Matsuura, Katsuhisa; Yamato, Masayuki; Takeda, Naoya; Okano, Teruo.

    In: Biomacromolecules, Vol. 14, No. 10, 14.10.2013, p. 3423-3433.

    Research output: Contribution to journalArticle

    Nagase, K, Hatakeyama, Y, Shimizu, T, Matsuura, K, Yamato, M, Takeda, N & Okano, T 2013, 'Hydrophobized thermoresponsive copolymer brushes for cell separation by multistep temperature change', Biomacromolecules, vol. 14, no. 10, pp. 3423-3433. https://doi.org/10.1021/bm4006722
    Nagase, Kenichi ; Hatakeyama, Yuri ; Shimizu, Tatsuya ; Matsuura, Katsuhisa ; Yamato, Masayuki ; Takeda, Naoya ; Okano, Teruo. / Hydrophobized thermoresponsive copolymer brushes for cell separation by multistep temperature change. In: Biomacromolecules. 2013 ; Vol. 14, No. 10. pp. 3423-3433.
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