Hypomyelination phenotype caused by impaired differentiation of oligodendrocytes in Emx1-cre mediated Cdk5 conditional knockout mice

Xiaojuan He, Satoru Takahashi, Hiromi Suzuki, Tsutomu Hashikawa, Ashok B. Kulkarni, Katsuhiko Mikoshiba, Toshio Ohshima

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Cyclin-dependent kinase 5 (Cdk5) plays a pivotal role in neuronal migration and differentiation, and in axonal elongation. Although many studies have been conducted to analyze neuronal functions of Cdk5, its kinase activity has also been reported during oligodendrocyte differentiation, which suggests Cdk5 may play an important role in oligodendrocytes. Here, we describe a hypomyelination phenotype observed in Emx1-cre mediated Cdk5 conditional knockout (cKO) mice (Emx1-cKO), in which the Cdk5 gene was deleted in neurons, astrocytes and oligodendrocyte -lineage cells. In contrast, the Cdk5 gene in CaMKII cKO mice was deleted only in neurons. Because the development of mature oligodendrocytes from oligodendrocyte precursor cells is a complex process, we performed in situ hybridization using markers for the oligodendrocyte precursor cell and for the differentiated oligodendrocyte. Our results indicate that hypomyelination in Emx1-cKO is due to the impaired differentiation of oligodendrocytes, rather than to the proliferation or migration of their precursors. The present study confirmed the in vivo role of Cdk5 in oligodendrocyte differentiation.

    Original languageEnglish
    Pages (from-to)1293-1303
    Number of pages11
    JournalNeurochemical Research
    Volume36
    Issue number7
    DOIs
    Publication statusPublished - 2011 Jul

    Fingerprint

    Cyclin-Dependent Kinase 5
    Oligodendroglia
    Knockout Mice
    Phenotype
    Neurons
    Genes
    Calcium-Calmodulin-Dependent Protein Kinase Type 2
    Elongation
    Phosphotransferases
    Astrocytes
    In Situ Hybridization

    Keywords

    • Conditional knockout mice
    • Cyclin-dependent kinase 5
    • Hypomyelination
    • Oligodendrocyte differentiation

    ASJC Scopus subject areas

    • Cellular and Molecular Neuroscience
    • Biochemistry

    Cite this

    Hypomyelination phenotype caused by impaired differentiation of oligodendrocytes in Emx1-cre mediated Cdk5 conditional knockout mice. / He, Xiaojuan; Takahashi, Satoru; Suzuki, Hiromi; Hashikawa, Tsutomu; Kulkarni, Ashok B.; Mikoshiba, Katsuhiko; Ohshima, Toshio.

    In: Neurochemical Research, Vol. 36, No. 7, 07.2011, p. 1293-1303.

    Research output: Contribution to journalArticle

    He, Xiaojuan ; Takahashi, Satoru ; Suzuki, Hiromi ; Hashikawa, Tsutomu ; Kulkarni, Ashok B. ; Mikoshiba, Katsuhiko ; Ohshima, Toshio. / Hypomyelination phenotype caused by impaired differentiation of oligodendrocytes in Emx1-cre mediated Cdk5 conditional knockout mice. In: Neurochemical Research. 2011 ; Vol. 36, No. 7. pp. 1293-1303.
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