Abstract
Cyclin-dependent kinase 5 (Cdk5) plays a pivotal role in neuronal migration and differentiation, and in axonal elongation. Although many studies have been conducted to analyze neuronal functions of Cdk5, its kinase activity has also been reported during oligodendrocyte differentiation, which suggests Cdk5 may play an important role in oligodendrocytes. Here, we describe a hypomyelination phenotype observed in Emx1-cre mediated Cdk5 conditional knockout (cKO) mice (Emx1-cKO), in which the Cdk5 gene was deleted in neurons, astrocytes and oligodendrocyte -lineage cells. In contrast, the Cdk5 gene in CaMKII cKO mice was deleted only in neurons. Because the development of mature oligodendrocytes from oligodendrocyte precursor cells is a complex process, we performed in situ hybridization using markers for the oligodendrocyte precursor cell and for the differentiated oligodendrocyte. Our results indicate that hypomyelination in Emx1-cKO is due to the impaired differentiation of oligodendrocytes, rather than to the proliferation or migration of their precursors. The present study confirmed the in vivo role of Cdk5 in oligodendrocyte differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 1293-1303 |
| Number of pages | 11 |
| Journal | Neurochemical Research |
| Volume | 36 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2011 Jul |
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Keywords
- Conditional knockout mice
- Cyclin-dependent kinase 5
- Hypomyelination
- Oligodendrocyte differentiation
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Biochemistry
Cite this
Hypomyelination phenotype caused by impaired differentiation of oligodendrocytes in Emx1-cre mediated Cdk5 conditional knockout mice. / He, Xiaojuan; Takahashi, Satoru; Suzuki, Hiromi; Hashikawa, Tsutomu; Kulkarni, Ashok B.; Mikoshiba, Katsuhiko; Ohshima, Toshio.
In: Neurochemical Research, Vol. 36, No. 7, 07.2011, p. 1293-1303.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hypomyelination phenotype caused by impaired differentiation of oligodendrocytes in Emx1-cre mediated Cdk5 conditional knockout mice
AU - He, Xiaojuan
AU - Takahashi, Satoru
AU - Suzuki, Hiromi
AU - Hashikawa, Tsutomu
AU - Kulkarni, Ashok B.
AU - Mikoshiba, Katsuhiko
AU - Ohshima, Toshio
PY - 2011/7
Y1 - 2011/7
N2 - Cyclin-dependent kinase 5 (Cdk5) plays a pivotal role in neuronal migration and differentiation, and in axonal elongation. Although many studies have been conducted to analyze neuronal functions of Cdk5, its kinase activity has also been reported during oligodendrocyte differentiation, which suggests Cdk5 may play an important role in oligodendrocytes. Here, we describe a hypomyelination phenotype observed in Emx1-cre mediated Cdk5 conditional knockout (cKO) mice (Emx1-cKO), in which the Cdk5 gene was deleted in neurons, astrocytes and oligodendrocyte -lineage cells. In contrast, the Cdk5 gene in CaMKII cKO mice was deleted only in neurons. Because the development of mature oligodendrocytes from oligodendrocyte precursor cells is a complex process, we performed in situ hybridization using markers for the oligodendrocyte precursor cell and for the differentiated oligodendrocyte. Our results indicate that hypomyelination in Emx1-cKO is due to the impaired differentiation of oligodendrocytes, rather than to the proliferation or migration of their precursors. The present study confirmed the in vivo role of Cdk5 in oligodendrocyte differentiation.
AB - Cyclin-dependent kinase 5 (Cdk5) plays a pivotal role in neuronal migration and differentiation, and in axonal elongation. Although many studies have been conducted to analyze neuronal functions of Cdk5, its kinase activity has also been reported during oligodendrocyte differentiation, which suggests Cdk5 may play an important role in oligodendrocytes. Here, we describe a hypomyelination phenotype observed in Emx1-cre mediated Cdk5 conditional knockout (cKO) mice (Emx1-cKO), in which the Cdk5 gene was deleted in neurons, astrocytes and oligodendrocyte -lineage cells. In contrast, the Cdk5 gene in CaMKII cKO mice was deleted only in neurons. Because the development of mature oligodendrocytes from oligodendrocyte precursor cells is a complex process, we performed in situ hybridization using markers for the oligodendrocyte precursor cell and for the differentiated oligodendrocyte. Our results indicate that hypomyelination in Emx1-cKO is due to the impaired differentiation of oligodendrocytes, rather than to the proliferation or migration of their precursors. The present study confirmed the in vivo role of Cdk5 in oligodendrocyte differentiation.
KW - Conditional knockout mice
KW - Cyclin-dependent kinase 5
KW - Hypomyelination
KW - Oligodendrocyte differentiation
UR - http://www.scopus.com/inward/record.url?scp=79959908129&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79959908129&partnerID=8YFLogxK
U2 - 10.1007/s11064-010-0391-0
DO - 10.1007/s11064-010-0391-0
M3 - Article
C2 - 21210220
AN - SCOPUS:79959908129
VL - 36
SP - 1293
EP - 1303
JO - Neurochemical Research
JF - Neurochemical Research
SN - 0364-3190
IS - 7
ER -