Hypomyelination phenotype caused by impaired differentiation of oligodendrocytes in Emx1-cre mediated Cdk5 conditional knockout mice

Xiaojuan He, Satoru Takahashi, Hiromi Suzuki, Tsutomu Hashikawa, Ashok B. Kulkarni, Katsuhiko Mikoshiba, Toshio Ohshima

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Cyclin-dependent kinase 5 (Cdk5) plays a pivotal role in neuronal migration and differentiation, and in axonal elongation. Although many studies have been conducted to analyze neuronal functions of Cdk5, its kinase activity has also been reported during oligodendrocyte differentiation, which suggests Cdk5 may play an important role in oligodendrocytes. Here, we describe a hypomyelination phenotype observed in Emx1-cre mediated Cdk5 conditional knockout (cKO) mice (Emx1-cKO), in which the Cdk5 gene was deleted in neurons, astrocytes and oligodendrocyte -lineage cells. In contrast, the Cdk5 gene in CaMKII cKO mice was deleted only in neurons. Because the development of mature oligodendrocytes from oligodendrocyte precursor cells is a complex process, we performed in situ hybridization using markers for the oligodendrocyte precursor cell and for the differentiated oligodendrocyte. Our results indicate that hypomyelination in Emx1-cKO is due to the impaired differentiation of oligodendrocytes, rather than to the proliferation or migration of their precursors. The present study confirmed the in vivo role of Cdk5 in oligodendrocyte differentiation.

Original languageEnglish
Pages (from-to)1293-1303
Number of pages11
JournalNeurochemical Research
Volume36
Issue number7
DOIs
Publication statusPublished - 2011 Jul

Keywords

  • Conditional knockout mice
  • Cyclin-dependent kinase 5
  • Hypomyelination
  • Oligodendrocyte differentiation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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