Hypothalamic expression and moonlight-independent changes of Cry3 and Per4 implicate their roles in lunar clock oscillators of the lunar-responsive goldlined spinefoot

Riko Toda, Keiko Okano, Yuki Takeuchi, Chihiro Yamauchi, Masato Fukushiro, Akihiro Takemura, Toshiyuki Okano

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Lunar cycle-associated physiology has been found in a wide variety of organisms. Studies suggest the presence of a circalunar clock in some animals, but the location of the lunar clock is unclear. We previously found lunar-associated expression of transcripts for Cryptochrome3 gene (SgCry3) in the brain of a lunar phase-responsive fish, the Goldlined spinefoot (Siganus guttatus). Then we proposed a photoperiodic model for the lunar phase response, in which SgCry3 might function as a phase-specific light response gene and/or an oscillatory factor in unidentified circalunar clock. In this study, we have developed an anti-SgCRY3 antibody to identify SgCRY3-immunoreactive cells in the brain. We found immunoreactions in the subependymal cells located in the mediobasal region of the diencephalon, a crucial site for photoperiodic seasonal responses in birds. For further assessment of the lunar-responding mechanism and the circalunar clock, we investigated mRNA levels of Cry3 as well as those of the other clock(-related) genes, Period (Per2 and Per4), in S. guttatus reared under nocturnal moonlight interruption or natural conditions. Not only SgCry3 but SgPer4 mRNA levels showed lunar phase-dependent variations in the diencephalon without depending on light condition during the night. These results suggest that the expressions of SgCry3 and SgPer4 are not directly regulated by moonlight stimulation but endogenously mediated in the brain, and implicate that circadian clock(-related) genes may be involved in the circalunar clock locating within the mediobasal region of the diencephalon.

    Original languageEnglish
    Article numbere109119
    JournalPLoS One
    Volume9
    Issue number10
    DOIs
    Publication statusPublished - 2014 Oct 1

    Fingerprint

    Diencephalon
    Clocks
    brain
    Genes
    Brain
    genes
    Siganus
    Light
    Messenger RNA
    Circadian Clocks
    circadian rhythm
    Birds
    Anti-Idiotypic Antibodies
    Fishes
    physiology
    cells
    Physiology
    antibodies
    birds
    organisms

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Hypothalamic expression and moonlight-independent changes of Cry3 and Per4 implicate their roles in lunar clock oscillators of the lunar-responsive goldlined spinefoot. / Toda, Riko; Okano, Keiko; Takeuchi, Yuki; Yamauchi, Chihiro; Fukushiro, Masato; Takemura, Akihiro; Okano, Toshiyuki.

    In: PLoS One, Vol. 9, No. 10, e109119, 01.10.2014.

    Research output: Contribution to journalArticle

    Toda, Riko ; Okano, Keiko ; Takeuchi, Yuki ; Yamauchi, Chihiro ; Fukushiro, Masato ; Takemura, Akihiro ; Okano, Toshiyuki. / Hypothalamic expression and moonlight-independent changes of Cry3 and Per4 implicate their roles in lunar clock oscillators of the lunar-responsive goldlined spinefoot. In: PLoS One. 2014 ; Vol. 9, No. 10.
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