Identification and characterization of Xenopus laevis homologs of mammalian TRAF6 and its binding protein TIFA

Jun Ichiro Inoue, Shigenori Yagi, Kosuke Ishikawa, Sakura Azuma, Shuntaro Ikawa, Kentaro Senba

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) transduces signals from members of the TNFR superfamily and the Toll/IL-1R family, leading to activation of transcription factors such as NFκB and AP-1. Genetic disruption of the TRAF6 gene in mice results in various developmental abnormalities during embryogenesis, including osteopetrosis, failure of neural tube closure, defective formation of skin appendices, absence of lymph nodes, and absence of mature thymic epithelial cells. To clarify the effect of TRAF6 in development, we previously identified a TRAF-interacting protein with a forkhead-associated domain (TIFA), which binds and activates TRAF6 upon extracellular stimulation. To understand the physiological roles of TRAF6 and TIFA in early development, we studied these genes in Xenopus laevis. Here, we describe identification of X. laevis homologs of mammalian TRAF6 (XTRAF6) and TIFA (XTIFA). As was the case for the mammalian homologs, overexpression of XTRAF6 or XTIFA activated NFκB, whereas XTIFA carrying a mutation that abolishes XTRAF6 binding failed to activate NFκB, suggesting that XTIFA activates NFκB by binding to XTRAF6. XTIFA and XTRAF6 mRNAs were expressed at similar levels in zygotes from the neurula stage and then increased. Whole-mount in situ hybridization revealed that XTRAF6 mRNA was expressed in the head region and neural tube during the neurula stage, and the expression expanded to the pharyngeal apparatus during the tailbud stage. This localization is consistent with the defective neural tube closure and abnormal thymus organogenesis observed in TRAF6-deficient mice. Our results suggest possible cooperation between XTRAF6 and XTIFA during embryogenesis.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalGene
Volume358
Issue number1-2
DOIs
Publication statusPublished - 2005 Sep 26
Externally publishedYes

Fingerprint

Neural Tube
Xenopus laevis
Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
Carrier Proteins
Embryonic Development
Osteopetrosis
Messenger RNA
Organogenesis
Zygote
Tumor Necrosis Factor Receptors
Transcription Factor AP-1
Thymus Gland
Genes
In Situ Hybridization
Transcription Factors
Lymph Nodes
Epithelial Cells
Head
Skin
Mutation

Keywords

  • Forkhead-associated domain
  • Neural tube
  • NFκB
  • Pharyngeal apparatus
  • Whole-mount in situ hybridization

ASJC Scopus subject areas

  • Genetics

Cite this

Identification and characterization of Xenopus laevis homologs of mammalian TRAF6 and its binding protein TIFA. / Inoue, Jun Ichiro; Yagi, Shigenori; Ishikawa, Kosuke; Azuma, Sakura; Ikawa, Shuntaro; Senba, Kentaro.

In: Gene, Vol. 358, No. 1-2, 26.09.2005, p. 53-59.

Research output: Contribution to journalArticle

Inoue, Jun Ichiro ; Yagi, Shigenori ; Ishikawa, Kosuke ; Azuma, Sakura ; Ikawa, Shuntaro ; Senba, Kentaro. / Identification and characterization of Xenopus laevis homologs of mammalian TRAF6 and its binding protein TIFA. In: Gene. 2005 ; Vol. 358, No. 1-2. pp. 53-59.
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