Identification and Expression Analysis of the Zebrafish Homologs of the ceramide synthase Gene Family

Mirco Brondolin, Susanne Berger, Michael Reinke, Hideomi Tanaka, Toshio Ohshima, Bernhard Fu, Michael Hoch

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Background: Sphingolipids represent a major class of lipids which both serve as structural components of membranes and as bioactive molecules involved in lipid signaling. Ceramide synthases (cers) reside in the center of sphingolipid metabolism by producing ceramide through de novo synthesis or degradative pathways. While the six mammalian cers family members have been extensively studied in cell culture and in adult tissues, a systematic analysis of cers expression and function during embryogenesis is still lacking. Results: Using bioinformatic and phylogenetic analysis, we identified nine highly conserved homologs of the vertebrate cers gene family in the zebrafish genome. A systematic expression analysis throughout five developmental stages indicates that, whereas until 48 hours post fertilization most zebrafish cers homologs are expressed in distinct patterns, e.g., in the intermediate cell mass and the pronephric duct, they show a highly overlapping expression during later stages of embryonic development, mostprominently in the developing brain. Conclusions: In this study, the expression of the cers gene homologs is comprehensively analyzed for the first time during vertebrate embryogenesis. Our data indicate that each embryonic tissue has a unique profile of cers expression during zebrafish embryogenesis suggesting tissue-specific profiles of ceramides and their derivatives.

    Original languageEnglish
    Pages (from-to)189-200
    Number of pages12
    JournalDevelopmental Dynamics
    Volume242
    Issue number2
    DOIs
    Publication statusPublished - 2013 Feb

    Fingerprint

    Zebrafish
    Embryonic Development
    Sphingolipids
    Ceramides
    Genes
    Vertebrates
    Lipids
    Computational Biology
    Fertilization
    Cell Culture Techniques
    Genome
    Gene Expression
    Membranes
    dihydroceramide desaturase
    Brain

    Keywords

    • Ceramide synthase genes
    • Development
    • Zebrafish

    ASJC Scopus subject areas

    • Developmental Biology

    Cite this

    Identification and Expression Analysis of the Zebrafish Homologs of the ceramide synthase Gene Family. / Brondolin, Mirco; Berger, Susanne; Reinke, Michael; Tanaka, Hideomi; Ohshima, Toshio; Fu, Bernhard; Hoch, Michael.

    In: Developmental Dynamics, Vol. 242, No. 2, 02.2013, p. 189-200.

    Research output: Contribution to journalArticle

    Brondolin, Mirco ; Berger, Susanne ; Reinke, Michael ; Tanaka, Hideomi ; Ohshima, Toshio ; Fu, Bernhard ; Hoch, Michael. / Identification and Expression Analysis of the Zebrafish Homologs of the ceramide synthase Gene Family. In: Developmental Dynamics. 2013 ; Vol. 242, No. 2. pp. 189-200.
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