Identification and functional analysis of the gene for type I myosin in fission yeast

Mika Toya, Fumio Motegi, Kentaro Nakano, Issei Mabuchi, Masayuki Yamamoto

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background: Type I myosin is highly conserved among eukaryotes, and apparently plays important roles in a number of cellular processes. In the budding yeast, two myosin I species have been identified and their role in F-actin assembly has been inferred. Results: We cloned the fission yeast myo1 gene, which apparently encoded a myosin I protein. Disruption of myo1 was not lethal, but it caused growth retardation at high and low temperatures, sensitivity to a high concentration of KC1, and aberrance in cell morphology associated with an abnormal distribution of F-actin patches. An abnormal deposition of cell wall materials was also seen. Homothallic myo1Δ cells could mate, but heterothallic myo1Δ cells were poor in conjugation. Myo1p was necessary for the encapsulation of spores. The tail domain of Myo1p was pivotal for its function. Calmodulin could bind to Myo1p through the IQ domain at the neck. Conclusions: Myo1p appears to control the redistribution of F-actin patches during the cell cycle. Loss of Myo1p function is likely to slow down the actin assembly/disassembly process, which results in a failure of the actin cycle to catch up with other events in both the mitotic and meiotic cell cycles, including extension of the conjugation tubes.

Original languageEnglish
Pages (from-to)187-199
Number of pages13
JournalGenes to Cells
Volume6
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Myosin Type I
Schizosaccharomyces
Actins
Genes
Cell Cycle
Saccharomycetales
Calmodulin
Spores
Eukaryota
Cell Wall
Neck
Temperature
Growth
Proteins

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Identification and functional analysis of the gene for type I myosin in fission yeast. / Toya, Mika; Motegi, Fumio; Nakano, Kentaro; Mabuchi, Issei; Yamamoto, Masayuki.

In: Genes to Cells, Vol. 6, No. 3, 2001, p. 187-199.

Research output: Contribution to journalArticle

Toya, Mika ; Motegi, Fumio ; Nakano, Kentaro ; Mabuchi, Issei ; Yamamoto, Masayuki. / Identification and functional analysis of the gene for type I myosin in fission yeast. In: Genes to Cells. 2001 ; Vol. 6, No. 3. pp. 187-199.
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