Identification and functional characterization of liposome tubulation protein from magnetotactic bacteria

Masayoshi Tanaka, Atsushi Arakaki, Tadashi Matsunaga

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Magnetotactic bacteria synthesize intracellular magnetosomes that are comprised of membrane-enveloped magnetic crystals. In this study, to identify the early stages of magnetosome formation, we isolated magnetosomes containing small magnetite crystals and those containing regular-sized magnetite crystals from Magnetospirillum magneticum AMB-1. This was achieved by using a novel size fractionation technique, resulting in the identification of a characteristic protein (Amb1018/MamY) from the small magnetite crystal fraction. The gene encoding MamY was located in the magnetosome island. Like the previously reported membrane deformation proteins, such as bin/amphiphysin/Rvs (BAR) and the dynamin family proteins, recombinant MamY protein bound directly to the liposomes, causing them to form long tubules. We established a mamY gene deletion mutant (ΔmamY) and analysed MamY protein localization in it for functional characterization of the protein in vivo. The ΔmamY mutant was found to have expanded magnetosome vesicles and a greater number of small magnetite crystals relative to the wild-type strain, suggesting that the function of the MamY protein is to constrict the magnetosome membrane during magnetosome vesicle formation, following which, the magnetite crystals grow to maturity within them.

Original languageEnglish
Pages (from-to)480-488
Number of pages9
JournalMolecular Microbiology
Volume76
Issue number2
DOIs
Publication statusPublished - 2010
Externally publishedYes

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Magnetosomes
Ferrosoferric Oxide
Liposomes
Bacteria
Proteins
Magnetospirillum
Dynamins
Membranes
Gene Deletion
Recombinant Proteins
Membrane Proteins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Medicine(all)

Cite this

Identification and functional characterization of liposome tubulation protein from magnetotactic bacteria. / Tanaka, Masayoshi; Arakaki, Atsushi; Matsunaga, Tadashi.

In: Molecular Microbiology, Vol. 76, No. 2, 2010, p. 480-488.

Research output: Contribution to journalArticle

Tanaka, Masayoshi ; Arakaki, Atsushi ; Matsunaga, Tadashi. / Identification and functional characterization of liposome tubulation protein from magnetotactic bacteria. In: Molecular Microbiology. 2010 ; Vol. 76, No. 2. pp. 480-488.
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