Identification and heterologous expression of the actinoallolide biosynthetic gene cluster

Yuki Inahashi, Taro Shiraishi, Akira Take, Atsuko Matsumoto, Yōko Takahashi, Satoshi Ōmura, Tomohisa Kuzuyama, Takuji Nakashima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Actinoallolides are anti-trypanosomal macrolides isolated from the secondary metabolites of two endophytic actinomycete strains, Actinoallomurus fulvus MK10-036 and K09-0307. A putative actinoallolide biosynthetic gene cluster was predicted from the genome sequence of the strain K09-0307. The gene cluster spans a contiguous 53 kb DNA region that comprises seven genes encoding three PKSs (aalA1, aalA2, and aalA3), cytochrome P450 (aalB), acyl-CoA dehydrogenase (aalC), crotonyl-CoA reductase (aalD), and TetR family regulator (aalR). The entire gene cluster was cloned into a plasmid pYIK1 by assembling DNA fragments, which were obtained from two cosmids containing left and right parts of the gene cluster. Following the introduction of an ermE* promoter at 100bp upstream from the start codon of aalA1, the gene cluster was introduced into Streptomyces coelicolor M1152. Subsequent LC-MS analysis revealed production of actinoallolide A in the culture broth. Thus, the actinoallolide biosynthetic gene cluster was identified by heterologous expression in Streptomyces.

Original languageEnglish
Pages (from-to)749-752
Number of pages4
JournalJournal of Antibiotics
Volume71
Issue number8
DOIs
Publication statusPublished - 2018 Aug 1
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

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  • Cite this

    Inahashi, Y., Shiraishi, T., Take, A., Matsumoto, A., Takahashi, Y., Ōmura, S., Kuzuyama, T., & Nakashima, T. (2018). Identification and heterologous expression of the actinoallolide biosynthetic gene cluster. Journal of Antibiotics, 71(8), 749-752. https://doi.org/10.1038/s41429-018-0057-8