Identification and localization of gonadotropin-inhibitory hormone (GnIH) orthologs in the hypothalamus of the red-eared slider turtle, Trachemys scripta elegans

Kazuyoshi Ukena, Eiko Iwakoshi-Ukena, Tomohiro Osugi, Kazuyoshi Tsutsui

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Gonadotropin-inhibitory hormone (GnIH) was discovered in 2000 as a novel hypothalamic neuropeptide that inhibited gonadotropin release in the Japanese quail. GnIH and its orthologs have a common C-terminal LPXRFamide (X = L or Q) motif, and have been identified in vertebrates from agnathans to humans, apart from reptiles. In the present study, we characterized a cDNA encoding GnIH orthologs in the brain of the red-eared slider turtle. The deduced precursor protein consisted of 205 amino-acid residues, encoding three putative peptide sequences that included the LPXRFamide motif at their C-termini. In addition, the precursor sequence was most similar to those of avian species. Immunoaffinity purification combined with mass spectrometry confirmed that three mature peptides were produced in the brain. In situ hybridization and immunohistochemistry showed that turtle GnIH-containing cells were restricted to the periventricular hypothalamic nucleus. Immunoreactive fibers were densely distributed in the median eminence. Thus, GnIH and related peptides may act on the pituitary to regulate pituitary hormone release in turtles as well as other vertebrates.

    Original languageEnglish
    Pages (from-to)69-76
    Number of pages8
    JournalGeneral and Comparative Endocrinology
    Volume227
    DOIs
    Publication statusPublished - 2016 Feb 1

    Fingerprint

    Trachemys scripta
    Turtles
    gonadotropins
    hypothalamus
    Gonadotropins
    turtles
    Hypothalamus
    hormones
    Hormones
    peptides
    vertebrates
    Vertebrates
    gonadotropin release
    brain
    pituitary hormones
    neuropeptides
    Coturnix
    Median Eminence
    Peptides
    Reptiles

    Keywords

    • cDNA cloning
    • Gonadotropin-inhibitory hormone (GnIH)
    • Hypothalamus
    • Neuropeptide
    • Turtle

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Identification and localization of gonadotropin-inhibitory hormone (GnIH) orthologs in the hypothalamus of the red-eared slider turtle, Trachemys scripta elegans. / Ukena, Kazuyoshi; Iwakoshi-Ukena, Eiko; Osugi, Tomohiro; Tsutsui, Kazuyoshi.

    In: General and Comparative Endocrinology, Vol. 227, 01.02.2016, p. 69-76.

    Research output: Contribution to journalArticle

    @article{b45efdca68d64b84b1dc265738fcf863,
    title = "Identification and localization of gonadotropin-inhibitory hormone (GnIH) orthologs in the hypothalamus of the red-eared slider turtle, Trachemys scripta elegans",
    abstract = "Gonadotropin-inhibitory hormone (GnIH) was discovered in 2000 as a novel hypothalamic neuropeptide that inhibited gonadotropin release in the Japanese quail. GnIH and its orthologs have a common C-terminal LPXRFamide (X = L or Q) motif, and have been identified in vertebrates from agnathans to humans, apart from reptiles. In the present study, we characterized a cDNA encoding GnIH orthologs in the brain of the red-eared slider turtle. The deduced precursor protein consisted of 205 amino-acid residues, encoding three putative peptide sequences that included the LPXRFamide motif at their C-termini. In addition, the precursor sequence was most similar to those of avian species. Immunoaffinity purification combined with mass spectrometry confirmed that three mature peptides were produced in the brain. In situ hybridization and immunohistochemistry showed that turtle GnIH-containing cells were restricted to the periventricular hypothalamic nucleus. Immunoreactive fibers were densely distributed in the median eminence. Thus, GnIH and related peptides may act on the pituitary to regulate pituitary hormone release in turtles as well as other vertebrates.",
    keywords = "cDNA cloning, Gonadotropin-inhibitory hormone (GnIH), Hypothalamus, Neuropeptide, Turtle",
    author = "Kazuyoshi Ukena and Eiko Iwakoshi-Ukena and Tomohiro Osugi and Kazuyoshi Tsutsui",
    year = "2016",
    month = "2",
    day = "1",
    doi = "10.1016/j.ygcen.2015.06.009",
    language = "English",
    volume = "227",
    pages = "69--76",
    journal = "General and Comparative Endocrinology",
    issn = "0016-6480",
    publisher = "Academic Press Inc.",

    }

    TY - JOUR

    T1 - Identification and localization of gonadotropin-inhibitory hormone (GnIH) orthologs in the hypothalamus of the red-eared slider turtle, Trachemys scripta elegans

    AU - Ukena, Kazuyoshi

    AU - Iwakoshi-Ukena, Eiko

    AU - Osugi, Tomohiro

    AU - Tsutsui, Kazuyoshi

    PY - 2016/2/1

    Y1 - 2016/2/1

    N2 - Gonadotropin-inhibitory hormone (GnIH) was discovered in 2000 as a novel hypothalamic neuropeptide that inhibited gonadotropin release in the Japanese quail. GnIH and its orthologs have a common C-terminal LPXRFamide (X = L or Q) motif, and have been identified in vertebrates from agnathans to humans, apart from reptiles. In the present study, we characterized a cDNA encoding GnIH orthologs in the brain of the red-eared slider turtle. The deduced precursor protein consisted of 205 amino-acid residues, encoding three putative peptide sequences that included the LPXRFamide motif at their C-termini. In addition, the precursor sequence was most similar to those of avian species. Immunoaffinity purification combined with mass spectrometry confirmed that three mature peptides were produced in the brain. In situ hybridization and immunohistochemistry showed that turtle GnIH-containing cells were restricted to the periventricular hypothalamic nucleus. Immunoreactive fibers were densely distributed in the median eminence. Thus, GnIH and related peptides may act on the pituitary to regulate pituitary hormone release in turtles as well as other vertebrates.

    AB - Gonadotropin-inhibitory hormone (GnIH) was discovered in 2000 as a novel hypothalamic neuropeptide that inhibited gonadotropin release in the Japanese quail. GnIH and its orthologs have a common C-terminal LPXRFamide (X = L or Q) motif, and have been identified in vertebrates from agnathans to humans, apart from reptiles. In the present study, we characterized a cDNA encoding GnIH orthologs in the brain of the red-eared slider turtle. The deduced precursor protein consisted of 205 amino-acid residues, encoding three putative peptide sequences that included the LPXRFamide motif at their C-termini. In addition, the precursor sequence was most similar to those of avian species. Immunoaffinity purification combined with mass spectrometry confirmed that three mature peptides were produced in the brain. In situ hybridization and immunohistochemistry showed that turtle GnIH-containing cells were restricted to the periventricular hypothalamic nucleus. Immunoreactive fibers were densely distributed in the median eminence. Thus, GnIH and related peptides may act on the pituitary to regulate pituitary hormone release in turtles as well as other vertebrates.

    KW - cDNA cloning

    KW - Gonadotropin-inhibitory hormone (GnIH)

    KW - Hypothalamus

    KW - Neuropeptide

    KW - Turtle

    UR - http://www.scopus.com/inward/record.url?scp=84959338130&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84959338130&partnerID=8YFLogxK

    U2 - 10.1016/j.ygcen.2015.06.009

    DO - 10.1016/j.ygcen.2015.06.009

    M3 - Article

    VL - 227

    SP - 69

    EP - 76

    JO - General and Comparative Endocrinology

    JF - General and Comparative Endocrinology

    SN - 0016-6480

    ER -