Identification, localization and expression of LPXRFamide peptides, and melatonin-dependent induction of their precursor mRNA in the newt brain

Vishwajit Sur Chowdhury, Takayoshi Ubuka, Tomohiro Osugi, Taichi Shimura, Kazuyoshi Tsutsui

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    The existence of RFamide peptides with a C-terminal LPXRFamide (XZL or Q) motif has been identified in the brain of various vertebrate species. However, the presence of LPXRFamide peptides in the urodele brain is not yet known. In this study, we cloned a cDNA encoding the precursor of LPXRFamide peptides from the newt brain by a combination of 3' and 5' rapid amplification of cDNA ends. The deduced LPXRFamide peptide precursor consisted of 233 amino acid residues, encoding four putative LPXRFamide peptides. All the peptide sequences were flanked by a glycine C-terminal amidation signal and basic amino acid on each end as an endoproteolytic site. Mass spectrometric analyses detected a nonapeptide, two decapeptides and an octapeptide produced from the precursor polypeptide in the brain as endogenous ligands. In situ hybridization further revealed the cellular localization of newt LPXRFamide (nLPXRFa) precursor mRNA in the suprachiasmatic nucleus (SCN) in the newt hypothalamus. Immunocytochemistry showed a cluster of cell bodies restricted to the SCN and their terminals in the median eminence. To understand the regulatory mechanism of nLPXRFa peptide expression, we further analyzed the effect of melatonin on the expression of nLPXRFa precursor mRNA. Melatonin administration to newts increased the expression of nLPXRFa precursor mRNA in the diencephalon. These results indicate that the urodele hypothalamus possesses LPXRFamide peptides and the expression of LPXRFamide peptides is regulated by melatonin. The localization of nLPXRFa peptides further suggests that these peptides may be involved in the regulation of pituitary hormone release in newts.

    Original languageEnglish
    Pages (from-to)211-220
    Number of pages10
    JournalJournal of Endocrinology
    Volume209
    Issue number2
    DOIs
    Publication statusPublished - 2011 May

    Fingerprint

    Salamandridae
    RNA Precursors
    Melatonin
    Peptides
    Brain
    Suprachiasmatic Nucleus
    Hypothalamus
    Complementary DNA
    Basic Amino Acids
    Diencephalon
    Median Eminence
    Pituitary Hormones
    Glycine
    In Situ Hybridization
    Vertebrates
    Immunohistochemistry

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Identification, localization and expression of LPXRFamide peptides, and melatonin-dependent induction of their precursor mRNA in the newt brain. / Chowdhury, Vishwajit Sur; Ubuka, Takayoshi; Osugi, Tomohiro; Shimura, Taichi; Tsutsui, Kazuyoshi.

    In: Journal of Endocrinology, Vol. 209, No. 2, 05.2011, p. 211-220.

    Research output: Contribution to journalArticle

    Chowdhury, Vishwajit Sur ; Ubuka, Takayoshi ; Osugi, Tomohiro ; Shimura, Taichi ; Tsutsui, Kazuyoshi. / Identification, localization and expression of LPXRFamide peptides, and melatonin-dependent induction of their precursor mRNA in the newt brain. In: Journal of Endocrinology. 2011 ; Vol. 209, No. 2. pp. 211-220.
    @article{ceb0f87c58104df68c7a51f6c763550e,
    title = "Identification, localization and expression of LPXRFamide peptides, and melatonin-dependent induction of their precursor mRNA in the newt brain",
    abstract = "The existence of RFamide peptides with a C-terminal LPXRFamide (XZL or Q) motif has been identified in the brain of various vertebrate species. However, the presence of LPXRFamide peptides in the urodele brain is not yet known. In this study, we cloned a cDNA encoding the precursor of LPXRFamide peptides from the newt brain by a combination of 3' and 5' rapid amplification of cDNA ends. The deduced LPXRFamide peptide precursor consisted of 233 amino acid residues, encoding four putative LPXRFamide peptides. All the peptide sequences were flanked by a glycine C-terminal amidation signal and basic amino acid on each end as an endoproteolytic site. Mass spectrometric analyses detected a nonapeptide, two decapeptides and an octapeptide produced from the precursor polypeptide in the brain as endogenous ligands. In situ hybridization further revealed the cellular localization of newt LPXRFamide (nLPXRFa) precursor mRNA in the suprachiasmatic nucleus (SCN) in the newt hypothalamus. Immunocytochemistry showed a cluster of cell bodies restricted to the SCN and their terminals in the median eminence. To understand the regulatory mechanism of nLPXRFa peptide expression, we further analyzed the effect of melatonin on the expression of nLPXRFa precursor mRNA. Melatonin administration to newts increased the expression of nLPXRFa precursor mRNA in the diencephalon. These results indicate that the urodele hypothalamus possesses LPXRFamide peptides and the expression of LPXRFamide peptides is regulated by melatonin. The localization of nLPXRFa peptides further suggests that these peptides may be involved in the regulation of pituitary hormone release in newts.",
    author = "Chowdhury, {Vishwajit Sur} and Takayoshi Ubuka and Tomohiro Osugi and Taichi Shimura and Kazuyoshi Tsutsui",
    year = "2011",
    month = "5",
    doi = "10.1530/JOE-10-0494",
    language = "English",
    volume = "209",
    pages = "211--220",
    journal = "Journal of Endocrinology",
    issn = "0022-0795",
    publisher = "Society for Endocrinology",
    number = "2",

    }

    TY - JOUR

    T1 - Identification, localization and expression of LPXRFamide peptides, and melatonin-dependent induction of their precursor mRNA in the newt brain

    AU - Chowdhury, Vishwajit Sur

    AU - Ubuka, Takayoshi

    AU - Osugi, Tomohiro

    AU - Shimura, Taichi

    AU - Tsutsui, Kazuyoshi

    PY - 2011/5

    Y1 - 2011/5

    N2 - The existence of RFamide peptides with a C-terminal LPXRFamide (XZL or Q) motif has been identified in the brain of various vertebrate species. However, the presence of LPXRFamide peptides in the urodele brain is not yet known. In this study, we cloned a cDNA encoding the precursor of LPXRFamide peptides from the newt brain by a combination of 3' and 5' rapid amplification of cDNA ends. The deduced LPXRFamide peptide precursor consisted of 233 amino acid residues, encoding four putative LPXRFamide peptides. All the peptide sequences were flanked by a glycine C-terminal amidation signal and basic amino acid on each end as an endoproteolytic site. Mass spectrometric analyses detected a nonapeptide, two decapeptides and an octapeptide produced from the precursor polypeptide in the brain as endogenous ligands. In situ hybridization further revealed the cellular localization of newt LPXRFamide (nLPXRFa) precursor mRNA in the suprachiasmatic nucleus (SCN) in the newt hypothalamus. Immunocytochemistry showed a cluster of cell bodies restricted to the SCN and their terminals in the median eminence. To understand the regulatory mechanism of nLPXRFa peptide expression, we further analyzed the effect of melatonin on the expression of nLPXRFa precursor mRNA. Melatonin administration to newts increased the expression of nLPXRFa precursor mRNA in the diencephalon. These results indicate that the urodele hypothalamus possesses LPXRFamide peptides and the expression of LPXRFamide peptides is regulated by melatonin. The localization of nLPXRFa peptides further suggests that these peptides may be involved in the regulation of pituitary hormone release in newts.

    AB - The existence of RFamide peptides with a C-terminal LPXRFamide (XZL or Q) motif has been identified in the brain of various vertebrate species. However, the presence of LPXRFamide peptides in the urodele brain is not yet known. In this study, we cloned a cDNA encoding the precursor of LPXRFamide peptides from the newt brain by a combination of 3' and 5' rapid amplification of cDNA ends. The deduced LPXRFamide peptide precursor consisted of 233 amino acid residues, encoding four putative LPXRFamide peptides. All the peptide sequences were flanked by a glycine C-terminal amidation signal and basic amino acid on each end as an endoproteolytic site. Mass spectrometric analyses detected a nonapeptide, two decapeptides and an octapeptide produced from the precursor polypeptide in the brain as endogenous ligands. In situ hybridization further revealed the cellular localization of newt LPXRFamide (nLPXRFa) precursor mRNA in the suprachiasmatic nucleus (SCN) in the newt hypothalamus. Immunocytochemistry showed a cluster of cell bodies restricted to the SCN and their terminals in the median eminence. To understand the regulatory mechanism of nLPXRFa peptide expression, we further analyzed the effect of melatonin on the expression of nLPXRFa precursor mRNA. Melatonin administration to newts increased the expression of nLPXRFa precursor mRNA in the diencephalon. These results indicate that the urodele hypothalamus possesses LPXRFamide peptides and the expression of LPXRFamide peptides is regulated by melatonin. The localization of nLPXRFa peptides further suggests that these peptides may be involved in the regulation of pituitary hormone release in newts.

    UR - http://www.scopus.com/inward/record.url?scp=79955537660&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=79955537660&partnerID=8YFLogxK

    U2 - 10.1530/JOE-10-0494

    DO - 10.1530/JOE-10-0494

    M3 - Article

    C2 - 21325452

    AN - SCOPUS:79955537660

    VL - 209

    SP - 211

    EP - 220

    JO - Journal of Endocrinology

    JF - Journal of Endocrinology

    SN - 0022-0795

    IS - 2

    ER -