Identification of a cDNA encoding a novel amphibian growth hormone-releasing peptide and localization of its transcript

K. Sawada, K. Ukena, S. Kikuyama, Kazuyoshi Tsutsui

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Recently, we identified in the bullfrog brairi a novel neuropeptide with a C-terminal Leu-Pro-Leu-Arg-Phe-NH2 sequence. This amphibian neuropeptide was shown to stimulate growth hormone (GH) release in vitro and in vivo and so was designated frog GH-releasiug peptide (fGRP). In this study, we cloned a cDNA encoding fGRP from the bullfrog brain by a combinatiou of 3′ and 5′ rapid amplification of cDNA ends (RACE). The deduced fGRP precursor consisted of 221 amino acid residues, encoding one fGRP and three putative fCRP-related peptides that included Leu-Pro-Xaa-Arg-Phe-NH2 (Xaa = Leu or Gln) at their C-termini. All these peptide sequences were flanked by a glycine C-terminal amidation signal and a single basic amino acid on each end as an endoproteolytic site. Northern blot analysis detected a single band of approximately 1.0 kb, indicating that no alternatively spliced forms were present. Such an apparent migration was in agreement with the estimated length of the cDNA, 902 bp. In situ hybridization further revealed the cellular localization of fGRP mRNA in the suprachiasmatic nucleus in the hypothalamus. In addition to fGRP, its related peptides may be hypothalamic factors involved in pituitary hormone secretion.

Original languageEnglish
Pages (from-to)395-402
Number of pages8
JournalJournal of Endocrinology
Volume174
Issue number3
DOIs
Publication statusPublished - 2002 Sep
Externally publishedYes

Fingerprint

Amphibians
Complementary DNA
Anura
Peptides
Rana catesbeiana
Neuropeptides
leucylproline
Growth Hormone
gamma-aminobutyryl-2-methyltryptophyl-2-methyltryptophyl-2-methyltryptophyl-lysinamide
Basic Amino Acids
Suprachiasmatic Nucleus
Pituitary Hormones
Northern Blotting
Glycine
Hypothalamus
In Situ Hybridization
Amino Acids
Messenger RNA
Brain

ASJC Scopus subject areas

  • Endocrinology

Cite this

Identification of a cDNA encoding a novel amphibian growth hormone-releasing peptide and localization of its transcript. / Sawada, K.; Ukena, K.; Kikuyama, S.; Tsutsui, Kazuyoshi.

In: Journal of Endocrinology, Vol. 174, No. 3, 09.2002, p. 395-402.

Research output: Contribution to journalArticle

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