Identification of a novel Sry-related gene and its germ cell-specific expression

Emiko Osaki, Yukio Nishina, Johji Inazawa, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Miho Ohsugi, Tohru Tezuka, Mitsuaki Yoshida, Kentaro Senba

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Sox family proteins are characterized by a unique DNA-binding domain, a HMG box which shows at least 50% sequence similarity with mouse Sry, the sex-determining factor. At present almost 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development, Some are involved in human diseases, including sex reversal. Here we report the isolation of a novel member of the Sox gene family, Sox30, which may constitute a distinct subgroup of this family. Using a bacterially expressed DNA-binding domain of Sox30, we show that it is able to specifically recognize the ACAAT motif. Furthermore, Sox30 is capable of activating transcription from a synthetic promoter containing the ACAAT motif. The specific expression of Sox30 in normal testes, but not in maturing germ cell-deficient testes, suggests the involvement of Sox30 in differentiation of male germ cells. Mapping analyses revealed that the Sox30 gene is located on human chromosome 5 (5q33) and on mouse chromosome 11.

Original languageEnglish
Pages (from-to)2503-2510
Number of pages8
JournalNucleic Acids Research
Volume27
Issue number12
DOIs
Publication statusPublished - 1999 Jun 15
Externally publishedYes

Fingerprint

sry Genes
Germ Cells
HMG-Box Domains
Testis
Genes
Sex Factors
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 11
DNA
Human Chromosomes
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Osaki, E., Nishina, Y., Inazawa, J., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., ... Senba, K. (1999). Identification of a novel Sry-related gene and its germ cell-specific expression. Nucleic Acids Research, 27(12), 2503-2510. https://doi.org/10.1093/nar/27.12.2503

Identification of a novel Sry-related gene and its germ cell-specific expression. / Osaki, Emiko; Nishina, Yukio; Inazawa, Johji; Copeland, Neal G.; Gilbert, Debra J.; Jenkins, Nancy A.; Ohsugi, Miho; Tezuka, Tohru; Yoshida, Mitsuaki; Senba, Kentaro.

In: Nucleic Acids Research, Vol. 27, No. 12, 15.06.1999, p. 2503-2510.

Research output: Contribution to journalArticle

Osaki, E, Nishina, Y, Inazawa, J, Copeland, NG, Gilbert, DJ, Jenkins, NA, Ohsugi, M, Tezuka, T, Yoshida, M & Senba, K 1999, 'Identification of a novel Sry-related gene and its germ cell-specific expression', Nucleic Acids Research, vol. 27, no. 12, pp. 2503-2510. https://doi.org/10.1093/nar/27.12.2503
Osaki E, Nishina Y, Inazawa J, Copeland NG, Gilbert DJ, Jenkins NA et al. Identification of a novel Sry-related gene and its germ cell-specific expression. Nucleic Acids Research. 1999 Jun 15;27(12):2503-2510. https://doi.org/10.1093/nar/27.12.2503
Osaki, Emiko ; Nishina, Yukio ; Inazawa, Johji ; Copeland, Neal G. ; Gilbert, Debra J. ; Jenkins, Nancy A. ; Ohsugi, Miho ; Tezuka, Tohru ; Yoshida, Mitsuaki ; Senba, Kentaro. / Identification of a novel Sry-related gene and its germ cell-specific expression. In: Nucleic Acids Research. 1999 ; Vol. 27, No. 12. pp. 2503-2510.
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