Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs

Keita Iyori, Junzo Hisatsune, Tetsuji Kawakami, Sanae Shibata, Nobuo Murayama, Kaori Ide, Masahiko Nagata, Tsuneo Fukata, Toshiroh Iwasaki, Kenshiro Oshima, Masahira Hattori, Motoyuki Sugai, Koji Nishifuji

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Staphylococcal exfoliative toxins are involved in some cutaneous infections in mammals by targeting desmoglein 1 (Dsg1), a desmosomal cell-cell adhesion molecule. Recently, an exfoliative toxin gene (exi) was identified in Staphylococcus pseudintermedius isolated from canine pyoderma. The aim of this study was to identify novel exfoliative toxin genes in S. pseudintermedius. Here, we describe a novel orf in the genome of S. pseudintermedius isolated from canine impetigo, whose deduced amino acid sequence was homologous to that of the SHETB exfoliative toxin from Staphylococcus hyicus (70.4%). The ORF recombinant protein caused skin exfoliation and abolished cell surface staining of Dsg1 in canine skin. Moreover, the ORF protein degraded the recombinant extracellular domains of canine Dsg1, but not Dsg3, in vitro. PCR analysis revealed that the orf was present in 23.2% (23/99) of S. pseudintermedius isolates from dogs with superficial pyoderma exhibiting various clinical phenotypes, while the occurrence in S. pseudintermedius isolates from healthy dogs was 6.1% (3/49). In summary, this newly found orf in S. pseudintermedius encodes a novel exfoliative toxin, which targets a cell-cell adhesion molecule in canine epidermis and might be involved in a broad spectrum of canine pyoderma.

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalFEMS Microbiology Letters
Volume312
Issue number2
DOIs
Publication statusPublished - 2010 Nov
Externally publishedYes

Fingerprint

Exfoliatins
Pyoderma
Staphylococcus
Canidae
Desmoglein 1
Contagious Ecthyma
Dogs
Genes
Cell Adhesion Molecules
Recombinant Proteins
Skin
Open Reading Frames
Staphylococcus hyicus
Impetigo
Amino Acid Sequence Homology
Epidermis
Mammals
Genome
Staining and Labeling
Phenotype

Keywords

  • Canine
  • Desmoglein
  • Exfoliative toxin
  • Impetigo
  • Pyoderma
  • Staphylococcus pseudintermedius

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs. / Iyori, Keita; Hisatsune, Junzo; Kawakami, Tetsuji; Shibata, Sanae; Murayama, Nobuo; Ide, Kaori; Nagata, Masahiko; Fukata, Tsuneo; Iwasaki, Toshiroh; Oshima, Kenshiro; Hattori, Masahira; Sugai, Motoyuki; Nishifuji, Koji.

In: FEMS Microbiology Letters, Vol. 312, No. 2, 11.2010, p. 169-175.

Research output: Contribution to journalArticle

Iyori, K, Hisatsune, J, Kawakami, T, Shibata, S, Murayama, N, Ide, K, Nagata, M, Fukata, T, Iwasaki, T, Oshima, K, Hattori, M, Sugai, M & Nishifuji, K 2010, 'Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs', FEMS Microbiology Letters, vol. 312, no. 2, pp. 169-175. https://doi.org/10.1111/j.1574-6968.2010.02113.x
Iyori, Keita ; Hisatsune, Junzo ; Kawakami, Tetsuji ; Shibata, Sanae ; Murayama, Nobuo ; Ide, Kaori ; Nagata, Masahiko ; Fukata, Tsuneo ; Iwasaki, Toshiroh ; Oshima, Kenshiro ; Hattori, Masahira ; Sugai, Motoyuki ; Nishifuji, Koji. / Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs. In: FEMS Microbiology Letters. 2010 ; Vol. 312, No. 2. pp. 169-175.
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