Abstract
In the present study, we examined the effects of reagents that elevate cAMP concentration on protein phosphorylation in Drosophila brain cells. Application of forskolin or dopamine to brain cells that were prelabeled with 32Pi enhanced phosphorylation of a 25-kDa protein (p25a). To identify p25a, we isolated the protein, and partial amino acid sequences were analyzed. Database searches showed that p25a is a J-domain protein, dJDP that contains the J-domain found in the DnaJ (heat shock protein 40) family of molecular chaperones. (C) 2000 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 160-163 |
| Number of pages | 4 |
| Journal | Brain Research |
| Volume | 875 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 2000 Sep 1 |
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Keywords
- cAMP dependent protein kinase
- Drosophila
- Learning
- Phosphoprotein
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Identification of a protein phosphorylated by cAMP-dependent protein kinase in drosophila brain. / Inoue, Hiroko; Chikaoka, Yoko; Takahashi, Masami; Yoshioka, Tohru.
In: Brain Research, Vol. 875, No. 1-2, 01.09.2000, p. 160-163.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of a protein phosphorylated by cAMP-dependent protein kinase in drosophila brain
AU - Inoue, Hiroko
AU - Chikaoka, Yoko
AU - Takahashi, Masami
AU - Yoshioka, Tohru
PY - 2000/9/1
Y1 - 2000/9/1
N2 - In the present study, we examined the effects of reagents that elevate cAMP concentration on protein phosphorylation in Drosophila brain cells. Application of forskolin or dopamine to brain cells that were prelabeled with 32Pi enhanced phosphorylation of a 25-kDa protein (p25a). To identify p25a, we isolated the protein, and partial amino acid sequences were analyzed. Database searches showed that p25a is a J-domain protein, dJDP that contains the J-domain found in the DnaJ (heat shock protein 40) family of molecular chaperones. (C) 2000 Elsevier Science B.V.
AB - In the present study, we examined the effects of reagents that elevate cAMP concentration on protein phosphorylation in Drosophila brain cells. Application of forskolin or dopamine to brain cells that were prelabeled with 32Pi enhanced phosphorylation of a 25-kDa protein (p25a). To identify p25a, we isolated the protein, and partial amino acid sequences were analyzed. Database searches showed that p25a is a J-domain protein, dJDP that contains the J-domain found in the DnaJ (heat shock protein 40) family of molecular chaperones. (C) 2000 Elsevier Science B.V.
KW - cAMP dependent protein kinase
KW - Drosophila
KW - Learning
KW - Phosphoprotein
UR - http://www.scopus.com/inward/record.url?scp=0034284784&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034284784&partnerID=8YFLogxK
U2 - 10.1016/S0006-8993(00)02612-3
DO - 10.1016/S0006-8993(00)02612-3
M3 - Article
C2 - 10967311
AN - SCOPUS:0034284784
VL - 875
SP - 160
EP - 163
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -