Identification of a protein phosphorylated by cAMP-dependent protein kinase in drosophila brain

Hiroko Inoue; Yoko Chikaoka; Masami Takahashi; Tohru Yoshioka

doi:10.1016/S0006-8993(00)02612-3

Identification of a protein phosphorylated by cAMP-dependent protein kinase in drosophila brain

Hiroko Inoue, Yoko Chikaoka, Masami Takahashi, Tohru Yoshioka

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

In the present study, we examined the effects of reagents that elevate cAMP concentration on protein phosphorylation in Drosophila brain cells. Application of forskolin or dopamine to brain cells that were prelabeled with ³²Pi enhanced phosphorylation of a 25-kDa protein (p25a). To identify p25a, we isolated the protein, and partial amino acid sequences were analyzed. Database searches showed that p25a is a J-domain protein, dJDP that contains the J-domain found in the DnaJ (heat shock protein 40) family of molecular chaperones. (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	160-163
Number of pages	4
Journal	Brain Research
Volume	875
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(00)02612-3
Publication status	Published - 2000 Sep 1

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Keywords

cAMP dependent protein kinase
Drosophila
Learning
Phosphoprotein

ASJC Scopus subject areas

Neuroscience(all)

Cite this

Inoue, H., Chikaoka, Y., Takahashi, M., & Yoshioka, T. (2000). Identification of a protein phosphorylated by cAMP-dependent protein kinase in drosophila brain. Brain Research, 875(1-2), 160-163. https://doi.org/10.1016/S0006-8993(00)02612-3

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10.1016/S0006-8993(00)02612-3