Identification of a protein phosphorylated by cAMP-dependent protein kinase in drosophila brain

Hiroko Inoue, Yoko Chikaoka, Masami Takahashi, Tohru Yoshioka

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    In the present study, we examined the effects of reagents that elevate cAMP concentration on protein phosphorylation in Drosophila brain cells. Application of forskolin or dopamine to brain cells that were prelabeled with 32Pi enhanced phosphorylation of a 25-kDa protein (p25a). To identify p25a, we isolated the protein, and partial amino acid sequences were analyzed. Database searches showed that p25a is a J-domain protein, dJDP that contains the J-domain found in the DnaJ (heat shock protein 40) family of molecular chaperones. (C) 2000 Elsevier Science B.V.

    Original languageEnglish
    Pages (from-to)160-163
    Number of pages4
    JournalBrain Research
    Volume875
    Issue number1-2
    DOIs
    Publication statusPublished - 2000 Sep 1

    Fingerprint

    Cyclic AMP-Dependent Protein Kinases
    Drosophila
    HSP40 Heat-Shock Proteins
    Phosphorylation
    Molecular Chaperones
    Brain
    Colforsin
    Amino Acid Sequence
    Dopamine
    Proteins
    Databases
    Protein Domains
    Drosophila inturned protein

    Keywords

    • cAMP dependent protein kinase
    • Drosophila
    • Learning
    • Phosphoprotein

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Identification of a protein phosphorylated by cAMP-dependent protein kinase in drosophila brain. / Inoue, Hiroko; Chikaoka, Yoko; Takahashi, Masami; Yoshioka, Tohru.

    In: Brain Research, Vol. 875, No. 1-2, 01.09.2000, p. 160-163.

    Research output: Contribution to journalArticle

    Inoue, Hiroko ; Chikaoka, Yoko ; Takahashi, Masami ; Yoshioka, Tohru. / Identification of a protein phosphorylated by cAMP-dependent protein kinase in drosophila brain. In: Brain Research. 2000 ; Vol. 875, No. 1-2. pp. 160-163.
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