Abstract
Amelogenin expression is regulated in a cell-type-specific manner. Investigators have previously identified an enhancer element by using the 5ĝ2 flanking sequence of the amelogenin promoter. However, the cell-type-specific regulation of the amelogenin gene remains poorly understood. In some genes, the first intron regulates tissue-specific expression. We hypothesized that intron 1 is important for the cell-type-specific regulation of amelogenin expression. We identified a suppressor element between -74 and -464. We also found enhancer activity in intron 1. Additionally, we found that the suppressor element in the promoter region suppressed intron 1 enhancer activity. The suppressor and the enhancers acted in an ameloblast-like cell line, but not in HeLa cells. Mutation of the Oct-1 binding sites reversed the suppressor activity, suggesting that Oct-1 sites are essential for suppression. These results suggest that Oct-1 and intron 1 may contribute to cell-type-specific amelogenin expression.
| Original language | English |
|---|---|
| Pages (from-to) | 246-251 |
| Number of pages | 6 |
| Journal | Journal of Dental Research |
| Volume | 89 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2010 Mar |
| Externally published | Yes |
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Keywords
- Ameloblast.
- Amelogenin
- Cell-type-specific expression
- Intron
- Oct-1
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Identification of a suppressor element in the amelogenin promoter. / Xu, L.; Matsumoto, A.; Sasaki, A.; Harada, H.; Taniguchi, Akiyoshi.
In: Journal of Dental Research, Vol. 89, No. 3, 03.2010, p. 246-251.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of a suppressor element in the amelogenin promoter
AU - Xu, L.
AU - Matsumoto, A.
AU - Sasaki, A.
AU - Harada, H.
AU - Taniguchi, Akiyoshi
PY - 2010/3
Y1 - 2010/3
N2 - Amelogenin expression is regulated in a cell-type-specific manner. Investigators have previously identified an enhancer element by using the 5ĝ2 flanking sequence of the amelogenin promoter. However, the cell-type-specific regulation of the amelogenin gene remains poorly understood. In some genes, the first intron regulates tissue-specific expression. We hypothesized that intron 1 is important for the cell-type-specific regulation of amelogenin expression. We identified a suppressor element between -74 and -464. We also found enhancer activity in intron 1. Additionally, we found that the suppressor element in the promoter region suppressed intron 1 enhancer activity. The suppressor and the enhancers acted in an ameloblast-like cell line, but not in HeLa cells. Mutation of the Oct-1 binding sites reversed the suppressor activity, suggesting that Oct-1 sites are essential for suppression. These results suggest that Oct-1 and intron 1 may contribute to cell-type-specific amelogenin expression.
AB - Amelogenin expression is regulated in a cell-type-specific manner. Investigators have previously identified an enhancer element by using the 5ĝ2 flanking sequence of the amelogenin promoter. However, the cell-type-specific regulation of the amelogenin gene remains poorly understood. In some genes, the first intron regulates tissue-specific expression. We hypothesized that intron 1 is important for the cell-type-specific regulation of amelogenin expression. We identified a suppressor element between -74 and -464. We also found enhancer activity in intron 1. Additionally, we found that the suppressor element in the promoter region suppressed intron 1 enhancer activity. The suppressor and the enhancers acted in an ameloblast-like cell line, but not in HeLa cells. Mutation of the Oct-1 binding sites reversed the suppressor activity, suggesting that Oct-1 sites are essential for suppression. These results suggest that Oct-1 and intron 1 may contribute to cell-type-specific amelogenin expression.
KW - Ameloblast.
KW - Amelogenin
KW - Cell-type-specific expression
KW - Intron
KW - Oct-1
UR - http://www.scopus.com/inward/record.url?scp=77749260648&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77749260648&partnerID=8YFLogxK
U2 - 10.1177/0022034509355144
DO - 10.1177/0022034509355144
M3 - Article
C2 - 20044581
AN - SCOPUS:77749260648
VL - 89
SP - 246
EP - 251
JO - Journal of Dental Research
JF - Journal of Dental Research
SN - 0022-0345
IS - 3
ER -