Abstract
The N gene of Nicotiana tabacum cv. Samsun NN is a resistance gene for Tobacco mosaic virus. The transcription of the gene increases immediately after virus infection or transient expression of the elicitor p50, a helicase domain of the virus 126/180 K replicases. In this study, we cloned the upstream sequences of the N gene from Samsun NN and fused them to the GFP reporter gene for promoter assays. Agrobacterium-mediated transient gene expression in N. tabacum cv. Samsun nn lacking the N gene allowed us to evaluate promoter activity with or without the expression of p50 and/or N. Individual expression of p50 or N specifically stimulated the N promoter, although the stimulation by N was less prominent than that of p50. The coexpression of p50 and N resulted in higher stimulation of the N promoter than the individual expression. Through deletion and gain-of-function analyses of the upstream region, we identified a 20-bp elicitor-responsive sequence that was essential and sufficient for promoter stimulation by p50 and N. Based on these data, we discuss the possibility that the virus elicitor and N may mediate cooperatively the stimulation of the N promoter upon virus infection.
| Original language | English |
|---|---|
| Pages (from-to) | 101-108 |
| Number of pages | 8 |
| Journal | Physiological and Molecular Plant Pathology |
| Volume | 73 |
| Issue number | 4-5 |
| DOIs | |
| Publication status | Published - 2008 Nov |
| Externally published | Yes |
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Keywords
- Dof binding motif
- Elicitor
- HR
- Hypersensitive response
ASJC Scopus subject areas
- Plant Science
- Genetics
Cite this
Identification of a Tobacco mosaic virus elicitor-responsive sequence in the resistance gene N. / Haque, Ashraful; Sasaki, Nobumitsu; Kanegae, Hiromi; Mimori, Seisuke; Gao, Jun Shan; Nyunoya, Hiroshi.
In: Physiological and Molecular Plant Pathology, Vol. 73, No. 4-5, 11.2008, p. 101-108.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of a Tobacco mosaic virus elicitor-responsive sequence in the resistance gene N
AU - Haque, Ashraful
AU - Sasaki, Nobumitsu
AU - Kanegae, Hiromi
AU - Mimori, Seisuke
AU - Gao, Jun Shan
AU - Nyunoya, Hiroshi
PY - 2008/11
Y1 - 2008/11
N2 - The N gene of Nicotiana tabacum cv. Samsun NN is a resistance gene for Tobacco mosaic virus. The transcription of the gene increases immediately after virus infection or transient expression of the elicitor p50, a helicase domain of the virus 126/180 K replicases. In this study, we cloned the upstream sequences of the N gene from Samsun NN and fused them to the GFP reporter gene for promoter assays. Agrobacterium-mediated transient gene expression in N. tabacum cv. Samsun nn lacking the N gene allowed us to evaluate promoter activity with or without the expression of p50 and/or N. Individual expression of p50 or N specifically stimulated the N promoter, although the stimulation by N was less prominent than that of p50. The coexpression of p50 and N resulted in higher stimulation of the N promoter than the individual expression. Through deletion and gain-of-function analyses of the upstream region, we identified a 20-bp elicitor-responsive sequence that was essential and sufficient for promoter stimulation by p50 and N. Based on these data, we discuss the possibility that the virus elicitor and N may mediate cooperatively the stimulation of the N promoter upon virus infection.
AB - The N gene of Nicotiana tabacum cv. Samsun NN is a resistance gene for Tobacco mosaic virus. The transcription of the gene increases immediately after virus infection or transient expression of the elicitor p50, a helicase domain of the virus 126/180 K replicases. In this study, we cloned the upstream sequences of the N gene from Samsun NN and fused them to the GFP reporter gene for promoter assays. Agrobacterium-mediated transient gene expression in N. tabacum cv. Samsun nn lacking the N gene allowed us to evaluate promoter activity with or without the expression of p50 and/or N. Individual expression of p50 or N specifically stimulated the N promoter, although the stimulation by N was less prominent than that of p50. The coexpression of p50 and N resulted in higher stimulation of the N promoter than the individual expression. Through deletion and gain-of-function analyses of the upstream region, we identified a 20-bp elicitor-responsive sequence that was essential and sufficient for promoter stimulation by p50 and N. Based on these data, we discuss the possibility that the virus elicitor and N may mediate cooperatively the stimulation of the N promoter upon virus infection.
KW - Dof binding motif
KW - Elicitor
KW - HR
KW - Hypersensitive response
UR - http://www.scopus.com/inward/record.url?scp=67651122999&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67651122999&partnerID=8YFLogxK
U2 - 10.1016/j.pmpp.2009.03.001
DO - 10.1016/j.pmpp.2009.03.001
M3 - Article
AN - SCOPUS:67651122999
VL - 73
SP - 101
EP - 108
JO - Physiological and Molecular Plant Pathology
JF - Physiological and Molecular Plant Pathology
SN - 0885-5765
IS - 4-5
ER -