Identification of a Tobacco mosaic virus elicitor-responsive sequence in the resistance gene N

Ashraful Haque, Nobumitsu Sasaki, Hiromi Kanegae, Seisuke Mimori, Jun Shan Gao, Hiroshi Nyunoya

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The N gene of Nicotiana tabacum cv. Samsun NN is a resistance gene for Tobacco mosaic virus. The transcription of the gene increases immediately after virus infection or transient expression of the elicitor p50, a helicase domain of the virus 126/180 K replicases. In this study, we cloned the upstream sequences of the N gene from Samsun NN and fused them to the GFP reporter gene for promoter assays. Agrobacterium-mediated transient gene expression in N. tabacum cv. Samsun nn lacking the N gene allowed us to evaluate promoter activity with or without the expression of p50 and/or N. Individual expression of p50 or N specifically stimulated the N promoter, although the stimulation by N was less prominent than that of p50. The coexpression of p50 and N resulted in higher stimulation of the N promoter than the individual expression. Through deletion and gain-of-function analyses of the upstream region, we identified a 20-bp elicitor-responsive sequence that was essential and sufficient for promoter stimulation by p50 and N. Based on these data, we discuss the possibility that the virus elicitor and N may mediate cooperatively the stimulation of the N promoter upon virus infection.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalPhysiological and Molecular Plant Pathology
Volume73
Issue number4-5
DOIs
Publication statusPublished - 2008 Nov
Externally publishedYes

Fingerprint

Tobacco Mosaic Virus
Tobacco mosaic virus
promoter regions
Genes
Virus Diseases
viruses
genes
Tobacco
Nicotiana tabacum
Viruses
Agrobacterium
Reporter Genes
reporter genes
infection
elicitors
Gene Expression
transcription (genetics)
gene expression
assays

Keywords

  • Dof binding motif
  • Elicitor
  • HR
  • Hypersensitive response

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

Identification of a Tobacco mosaic virus elicitor-responsive sequence in the resistance gene N. / Haque, Ashraful; Sasaki, Nobumitsu; Kanegae, Hiromi; Mimori, Seisuke; Gao, Jun Shan; Nyunoya, Hiroshi.

In: Physiological and Molecular Plant Pathology, Vol. 73, No. 4-5, 11.2008, p. 101-108.

Research output: Contribution to journalArticle

Haque, Ashraful ; Sasaki, Nobumitsu ; Kanegae, Hiromi ; Mimori, Seisuke ; Gao, Jun Shan ; Nyunoya, Hiroshi. / Identification of a Tobacco mosaic virus elicitor-responsive sequence in the resistance gene N. In: Physiological and Molecular Plant Pathology. 2008 ; Vol. 73, No. 4-5. pp. 101-108.
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