Identification of cis-acting elements involved in an alternative splicing of the amyloid precursor protein (APP) gene

Asami Shibata, Masahira Hattori, Hideaki Suda, Yoshiyuki Sakaki

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Our previous study demonstrated that a cis-acting element for neuron-specific splicing of human amyloid precursor protein (APP) gene existed in an intron region flanking to exon 8. In this study, we extended the analysis by deletion and mutagenesis experiments and identified two distinct cis-acting elements which modulate the splicing of exon 8 of the APP gene in a sequence-dependent manner. Both elements are located at the just upstream region of the so-called branchpoint sequence, the first (ATGTTT) at -42 to -47 nt and the second (TTT) at -36 to -38 nt upstream from exon 8. Our study suggested that the first one is a positive element for the splicing of exon 8 and the second a negative one for the splicing.

Original languageEnglish
Pages (from-to)203-208
Number of pages6
JournalGene
Volume175
Issue number1-2
DOIs
Publication statusPublished - 1996 Oct 10
Externally publishedYes

Keywords

  • Alzheimer's disease
  • Exon skipping
  • Mini-APP gene
  • Neuron
  • Secondary structure

ASJC Scopus subject areas

  • Genetics

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