Identification of differentially expressed genes in hepatocyte/endothelial cell co-culture system

Goh Takayama, Akiyoshi Taniguchi, Teruo Okano

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Cell-cell communication between multiple-cell populations is believed to be important for the activation of many cellular functions. Previously, we showed that, in the rat hepatoma cell line Fao, grown in a layered co-culture system with a human umbilical vein endothelial cell (HUVEC) sheet, the expression of albumin and apolipoprotein A-I increased time-dependently for 10 days and was maintained at a significantly higher level than Fao without the HUVEC sheet. Because the gene-expression profile of hepatocytes and HUVECs under double-layered co-culture has not previously been elucidated, in the present study, we examined the difference in messenger ribonucleic acid expression between Fao or HUVEC monolayer cells and double-layered co-cultured Fao cells/HUVECs using suppression subtractive hybridization. More than 200 transcripts were differentially screened to ensure unique expression. The expression levels of genes in the co-cultured and monolayer cells were determined using SYBR Green I real-time reverse transcription polymerase chain reaction with species-specific primers. We found 23 genes that showed at least a 2-fold difference in expression level. Five hepatocyte-specific genes-α1-acidglycoprotein, α2-microglobulin, hepcidin, transferrin, and trausthyretin-were identified from the Fao cells. Two cell-surface protein genes-bone morphogenetic protein receptor type II and CD82-which may be related to cell-cell communication, showed greater expression in the HUVECs co-cultured with Fao cells. These results indicate that many hepatocyte and endothelial cell functions increase in intensity upon layered co-culture.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalTissue Engineering
Volume13
Issue number1
DOIs
Publication statusPublished - 2007 Jan
Externally publishedYes

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Endothelial cells
Coculture Techniques
Cell culture
Hepatocytes
Endothelial Cells
Cell Culture Techniques
Genes
Human Umbilical Vein Endothelial Cells
Monolayers
Cells
Proteins
Cell Communication
Cultured Cells
Polymerase chain reaction
Communication
Type II Bone Morphogenetic Protein Receptors
Transcription
RNA
Gene expression
Hepcidins

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

Cite this

Identification of differentially expressed genes in hepatocyte/endothelial cell co-culture system. / Takayama, Goh; Taniguchi, Akiyoshi; Okano, Teruo.

In: Tissue Engineering, Vol. 13, No. 1, 01.2007, p. 159-166.

Research output: Contribution to journalArticle

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