Identification of differentially expressed genes in senescence-accelerated mouse testes by suppression subtractive hybridization analysis

Takuya Chiba, Junjie Yao, Yoshikazu Higami, Isao Shimokawa, Masanori Hosokawa, Keiichi Higuchi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Senescence-accelerated mouse (SAM) strains constitute a model of accelerated senescence coupled with a short lifespan and the early development of various age-related disorders. To identify differential gene expression in testes between senescence-accelerated SAMP1 and control SAMR1 mice, we performed suppression subtractive hybridization. We observed that the expression of three genes related to cell proliferation (myosin regulatory light chain B, aldolase 1A isoform, and cytochrome c oxidase subunit VIc) were upregulated and four genes implicated in spermatogenesis were downregulated in SAMP1 mice. Asb-8, a member of ankyrin repeat-containing proteins, was abundantly expressed in the testes and downregulated in SAMP1. The other three downregulated genes (germ cell-specific gene 1, T-complex polypeptide 1b, and activator of cAMP responsive element modulator in testis) have been reported to regulate late-stage spermatogenesis. These gene expression profiles might explain the findings of early testicular maturation and rapid decline in the ability to produce spermatozoa with advancing age in SAMP1 mice.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalMammalian Genome
Volume18
Issue number2
DOIs
Publication statusPublished - 2007 Feb
Externally publishedYes

Fingerprint

Testis
Down-Regulation
Spermatogenesis
Genes
Cyclic AMP Response Element Modulator
Ankyrin Repeat
Gene Expression
Myosin Light Chains
Fructose-Bisphosphate Aldolase
Electron Transport Complex IV
Transcriptome
Germ Cells
Spermatozoa
Protein Isoforms
Cell Proliferation
Peptides
Subtractive Hybridization Techniques
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Identification of differentially expressed genes in senescence-accelerated mouse testes by suppression subtractive hybridization analysis. / Chiba, Takuya; Yao, Junjie; Higami, Yoshikazu; Shimokawa, Isao; Hosokawa, Masanori; Higuchi, Keiichi.

In: Mammalian Genome, Vol. 18, No. 2, 02.2007, p. 105-112.

Research output: Contribution to journalArticle

Chiba, Takuya ; Yao, Junjie ; Higami, Yoshikazu ; Shimokawa, Isao ; Hosokawa, Masanori ; Higuchi, Keiichi. / Identification of differentially expressed genes in senescence-accelerated mouse testes by suppression subtractive hybridization analysis. In: Mammalian Genome. 2007 ; Vol. 18, No. 2. pp. 105-112.
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