Identification of erythroid progenitors induced by erythropoietic activity in Xenopus laevis

Nami Nogawa-Kosaka, Tatsuhisa Sugai, Kazumichi Nagasawa, Yuta Tanizaki, Mizue Meguro, Youichi Aizawa, Shun Maekawa, Motoyasu Adachi, Ryota Kuroki, Takashi Kato

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    Oxygen is essential for the survival of animals. Red blood cells in the circulation, i.e. peripheral erythrocytes, are responsible for transporting oxygen to tissues. The regulation of erythropoiesis in vertebrates other than mammals is yet to be elucidated. Recently we identified erythropoietin, a primary regulator of erythropoiesis, in Xenopus laevis, which should enable us to identify target cells, including erythroid progenitors, and to investigate the production and development of erythroid cells in amphibians. Here, we established a semi-solid colony-forming assay in Xenopus laevis to clarify the existence of colony-forming unit-erythroid cells, the functional erythroid progenitors identified in vitro. Using this assay, we showed that recombinant xlEPO induces erythroid colony formation in vitro and detected an increased level of erythropoietin activity in blood serum during acute anemic stress. In addition, our study demonstrated the possible presence of multiple, non-x/EPO, factors in anemic serum supportive of erythroid colony formation. These results indicate that erythropoiesis mediated by erythropoietin is present in amphibian species and, furthermore, that the regulatory mechanisms controlling peripheral erythrocyte number may vary among vertebrates.

    Original languageEnglish
    Pages (from-to)921-927
    Number of pages7
    JournalJournal of Experimental Biology
    Volume214
    Issue number6
    DOIs
    Publication statusPublished - 2011 Mar

    Fingerprint

    erythropoiesis
    erythropoietin
    Erythropoiesis
    Xenopus laevis
    Erythropoietin
    Erythroid Cells
    Amphibians
    blood serum
    Vertebrates
    amphibians
    erythrocytes
    Erythrocytes
    vertebrates
    amphibian
    Oxygen
    oxygen
    serum
    Erythroid Precursor Cells
    vertebrate
    Erythrocyte Count

    Keywords

    • Anemia
    • Colony assay
    • EPO
    • Erythropoiesis
    • Phenylhydrazine
    • Xenopus laevis

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • Ecology, Evolution, Behavior and Systematics
    • Molecular Biology
    • Physiology
    • Insect Science
    • Aquatic Science

    Cite this

    Identification of erythroid progenitors induced by erythropoietic activity in Xenopus laevis. / Nogawa-Kosaka, Nami; Sugai, Tatsuhisa; Nagasawa, Kazumichi; Tanizaki, Yuta; Meguro, Mizue; Aizawa, Youichi; Maekawa, Shun; Adachi, Motoyasu; Kuroki, Ryota; Kato, Takashi.

    In: Journal of Experimental Biology, Vol. 214, No. 6, 03.2011, p. 921-927.

    Research output: Contribution to journalArticle

    Nogawa-Kosaka, N, Sugai, T, Nagasawa, K, Tanizaki, Y, Meguro, M, Aizawa, Y, Maekawa, S, Adachi, M, Kuroki, R & Kato, T 2011, 'Identification of erythroid progenitors induced by erythropoietic activity in Xenopus laevis', Journal of Experimental Biology, vol. 214, no. 6, pp. 921-927. https://doi.org/10.1242/jeb.050286
    Nogawa-Kosaka N, Sugai T, Nagasawa K, Tanizaki Y, Meguro M, Aizawa Y et al. Identification of erythroid progenitors induced by erythropoietic activity in Xenopus laevis. Journal of Experimental Biology. 2011 Mar;214(6):921-927. https://doi.org/10.1242/jeb.050286
    Nogawa-Kosaka, Nami ; Sugai, Tatsuhisa ; Nagasawa, Kazumichi ; Tanizaki, Yuta ; Meguro, Mizue ; Aizawa, Youichi ; Maekawa, Shun ; Adachi, Motoyasu ; Kuroki, Ryota ; Kato, Takashi. / Identification of erythroid progenitors induced by erythropoietic activity in Xenopus laevis. In: Journal of Experimental Biology. 2011 ; Vol. 214, No. 6. pp. 921-927.
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