Identification of erythropoietin-induced microRNAs in haematopoietic cells during erythroid differentiation

Nobuyoshi Kosaka, Keiichi Sugiura, Yusuke Yamamoto, Yusuke Yoshioka, Hiroshi Miyazaki, Norio Komatsu, Takahiro Ochiya, Takashi Kato

    Research output: Contribution to journalArticle

    34 Citations (Scopus)

    Abstract

    MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression through mRNA degradation or translation inhibition. It has not yet been clearly elucidated whether miRNAs participate in haematopoietic processes such as cell differentiation, apoptosis and maintenance of adequate levels of circulating blood cells. A human miRNA microarray was used to analyze miRNA expression in the erythropoietin-dependent cell line UT-7/EPO compared to other factor-dependent UT-7 cell lines. Among 324 human miRNAs, MIRN188, MIRN362 and MIRN210 levels were significantly elevated in UT-7/EPO cells, and stimulation with EPO in UT-7 cells increased the level of these three miRNAs. Notably, knockdown of MIRN210 in UT-7/EPO cells led to apoptosis. In mouse fetal liver cells, MIRN210 expression was twofold higher in TER-119-positive cells than in TER-119-negative cells. The expression of MIRN210 was elevated during erythroid maturation in vitro. These data suggest MIRN210 to be a member a new class of regulatory miRNAs that might play an important role in erythroid maturation.

    Original languageEnglish
    Pages (from-to)293-300
    Number of pages8
    JournalBritish Journal of Haematology
    Volume142
    Issue number2
    DOIs
    Publication statusPublished - 2008 Jul

    Fingerprint

    Erythroid Cells
    Erythropoietin
    MicroRNAs
    Apoptosis
    Cell Line
    Small Untranslated RNA
    RNA Stability
    Protein Biosynthesis
    Cell Differentiation
    Blood Cells
    Gene Expression
    Liver

    Keywords

    • Apoptosis
    • Erythrocyte
    • Erythropoietin
    • MIRN210
    • MiRNA

    ASJC Scopus subject areas

    • Hematology

    Cite this

    Identification of erythropoietin-induced microRNAs in haematopoietic cells during erythroid differentiation. / Kosaka, Nobuyoshi; Sugiura, Keiichi; Yamamoto, Yusuke; Yoshioka, Yusuke; Miyazaki, Hiroshi; Komatsu, Norio; Ochiya, Takahiro; Kato, Takashi.

    In: British Journal of Haematology, Vol. 142, No. 2, 07.2008, p. 293-300.

    Research output: Contribution to journalArticle

    Kosaka, Nobuyoshi ; Sugiura, Keiichi ; Yamamoto, Yusuke ; Yoshioka, Yusuke ; Miyazaki, Hiroshi ; Komatsu, Norio ; Ochiya, Takahiro ; Kato, Takashi. / Identification of erythropoietin-induced microRNAs in haematopoietic cells during erythroid differentiation. In: British Journal of Haematology. 2008 ; Vol. 142, No. 2. pp. 293-300.
    @article{58c05e3c2cd84d92845ba53dadb542f8,
    title = "Identification of erythropoietin-induced microRNAs in haematopoietic cells during erythroid differentiation",
    abstract = "MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression through mRNA degradation or translation inhibition. It has not yet been clearly elucidated whether miRNAs participate in haematopoietic processes such as cell differentiation, apoptosis and maintenance of adequate levels of circulating blood cells. A human miRNA microarray was used to analyze miRNA expression in the erythropoietin-dependent cell line UT-7/EPO compared to other factor-dependent UT-7 cell lines. Among 324 human miRNAs, MIRN188, MIRN362 and MIRN210 levels were significantly elevated in UT-7/EPO cells, and stimulation with EPO in UT-7 cells increased the level of these three miRNAs. Notably, knockdown of MIRN210 in UT-7/EPO cells led to apoptosis. In mouse fetal liver cells, MIRN210 expression was twofold higher in TER-119-positive cells than in TER-119-negative cells. The expression of MIRN210 was elevated during erythroid maturation in vitro. These data suggest MIRN210 to be a member a new class of regulatory miRNAs that might play an important role in erythroid maturation.",
    keywords = "Apoptosis, Erythrocyte, Erythropoietin, MIRN210, MiRNA",
    author = "Nobuyoshi Kosaka and Keiichi Sugiura and Yusuke Yamamoto and Yusuke Yoshioka and Hiroshi Miyazaki and Norio Komatsu and Takahiro Ochiya and Takashi Kato",
    year = "2008",
    month = "7",
    doi = "10.1111/j.1365-2141.2008.07151.x",
    language = "English",
    volume = "142",
    pages = "293--300",
    journal = "British Journal of Haematology",
    issn = "0007-1048",
    publisher = "Wiley-Blackwell",
    number = "2",

    }

    TY - JOUR

    T1 - Identification of erythropoietin-induced microRNAs in haematopoietic cells during erythroid differentiation

    AU - Kosaka, Nobuyoshi

    AU - Sugiura, Keiichi

    AU - Yamamoto, Yusuke

    AU - Yoshioka, Yusuke

    AU - Miyazaki, Hiroshi

    AU - Komatsu, Norio

    AU - Ochiya, Takahiro

    AU - Kato, Takashi

    PY - 2008/7

    Y1 - 2008/7

    N2 - MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression through mRNA degradation or translation inhibition. It has not yet been clearly elucidated whether miRNAs participate in haematopoietic processes such as cell differentiation, apoptosis and maintenance of adequate levels of circulating blood cells. A human miRNA microarray was used to analyze miRNA expression in the erythropoietin-dependent cell line UT-7/EPO compared to other factor-dependent UT-7 cell lines. Among 324 human miRNAs, MIRN188, MIRN362 and MIRN210 levels were significantly elevated in UT-7/EPO cells, and stimulation with EPO in UT-7 cells increased the level of these three miRNAs. Notably, knockdown of MIRN210 in UT-7/EPO cells led to apoptosis. In mouse fetal liver cells, MIRN210 expression was twofold higher in TER-119-positive cells than in TER-119-negative cells. The expression of MIRN210 was elevated during erythroid maturation in vitro. These data suggest MIRN210 to be a member a new class of regulatory miRNAs that might play an important role in erythroid maturation.

    AB - MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression through mRNA degradation or translation inhibition. It has not yet been clearly elucidated whether miRNAs participate in haematopoietic processes such as cell differentiation, apoptosis and maintenance of adequate levels of circulating blood cells. A human miRNA microarray was used to analyze miRNA expression in the erythropoietin-dependent cell line UT-7/EPO compared to other factor-dependent UT-7 cell lines. Among 324 human miRNAs, MIRN188, MIRN362 and MIRN210 levels were significantly elevated in UT-7/EPO cells, and stimulation with EPO in UT-7 cells increased the level of these three miRNAs. Notably, knockdown of MIRN210 in UT-7/EPO cells led to apoptosis. In mouse fetal liver cells, MIRN210 expression was twofold higher in TER-119-positive cells than in TER-119-negative cells. The expression of MIRN210 was elevated during erythroid maturation in vitro. These data suggest MIRN210 to be a member a new class of regulatory miRNAs that might play an important role in erythroid maturation.

    KW - Apoptosis

    KW - Erythrocyte

    KW - Erythropoietin

    KW - MIRN210

    KW - MiRNA

    UR - http://www.scopus.com/inward/record.url?scp=47249165678&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=47249165678&partnerID=8YFLogxK

    U2 - 10.1111/j.1365-2141.2008.07151.x

    DO - 10.1111/j.1365-2141.2008.07151.x

    M3 - Article

    C2 - 18492109

    AN - SCOPUS:47249165678

    VL - 142

    SP - 293

    EP - 300

    JO - British Journal of Haematology

    JF - British Journal of Haematology

    SN - 0007-1048

    IS - 2

    ER -