Identification of functional domains of the Escherichia coli SeqA protein

Norie Fujikawa, Hitoshi Kurumizaka, Mitsuyoshi Yamazoe, Sota Hiraga, Shigeyuki Yokoyama

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The Escherichia coli SeqA protein, a negative regulator of chromosomal DNA replication, prevents the overinitiation of replication within one cell cycle by binding to hemimethylated G-mA-T-C sequences in the replication origin, oriC. In addition to the hemimethylated DNA-binding activity, the SeqA protein has a self-association activity, which is also considered to be essential for its regulatory function in replication initiation. To study the functional domains responsible for the DNA-binding and self-association activities, we performed a deletion analysis of the SeqA protein and found that the N-terminal (amino acid residues 1-59) and the C-terminal (amino acid residues 71-181) regions form structurally distinct domains. The N-terminal domain, which is not involved in DNA binding, has the self-association activity. In contrast, the C-terminal domain, which lacks the self-association activity, specifically binds to the hemimethylated G-mA-T-C sequence. Therefore, two essential SeqA activities, self-association and DNA-binding, are independently performed by the structurally distinct N-terminal and C-terminal domains, respectively.

Original languageEnglish
Pages (from-to)699-705
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume300
Issue number3
DOIs
Publication statusPublished - 2003 Jan 17
Externally publishedYes

Fingerprint

Escherichia coli Proteins
Escherichia coli
DNA
Proteins
Amino Acids
Replication Origin
DNA Replication
Cell Cycle
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of functional domains of the Escherichia coli SeqA protein. / Fujikawa, Norie; Kurumizaka, Hitoshi; Yamazoe, Mitsuyoshi; Hiraga, Sota; Yokoyama, Shigeyuki.

In: Biochemical and Biophysical Research Communications, Vol. 300, No. 3, 17.01.2003, p. 699-705.

Research output: Contribution to journalArticle

Fujikawa, Norie ; Kurumizaka, Hitoshi ; Yamazoe, Mitsuyoshi ; Hiraga, Sota ; Yokoyama, Shigeyuki. / Identification of functional domains of the Escherichia coli SeqA protein. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 300, No. 3. pp. 699-705.
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