Identification of genetic and epigenetic similarities of SPHK1/Sphk1 in mammals

Takuya Imamura, Nanami Miyauchi-Senda, Satoshi Tanaka, Kunio Shiota

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In normal tissues, methylation of CpG islands is generally accepted to be limited to the inactive X-chromosome and imprinting clusters. Gene Sphk1 has shown complex organization, indicated by multiple alternative splicing and tissue-dependent DNA methylation within the limited area (T-DMR) of the CpG island in the rat. Comparisons among human, mouse and rat SPHK1/Sphk1 genomic DNA revealed five coding exons and association of a CpG island at the 5′ end in common. We also found two novel subtypes, for a total of eight mRNA subtypes generated through selective usage of untranslated first exons. A 38-bp region at the 5′-end of T-DMR is highly conserved. This restricted area is specifically hypomethylated in the brain. Here, we examine the complex genetic/epigenetic features of the SPHK1/Sphk1 CpG island, and suggest that the T-DMR is the core target for tissue-dependent CpG island methylation.

Original languageEnglish
Pages (from-to)1387-1393
Number of pages7
JournalJournal of Veterinary Medical Science
Volume66
Issue number11
DOIs
Publication statusPublished - 2004 Nov
Externally publishedYes

Keywords

  • Alternative splicing
  • CpG island
  • DNA methylation
  • Sphingosine kinase
  • Tissue specific

ASJC Scopus subject areas

  • veterinary(all)
  • Marketing

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