Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain

Motoyuki Shimizu, Keiichi Higuchi, Beth Bennett, Chen Xia, Tadao Tsuboyama, Soichiro Kasai, Takuya Chiba, Hiromi Fujisawa, Kumiko Kogishi, Haruo Kitado, Mitsutoshi Kimoto, Norikazu Takeda, Mutsumi Matsushita, Hideo Okumura, Tadao Serikawa, Takashi Nakamura, Thomas E. Johnson, Masanori Hosokawa

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The whole genome scan for quantitative trait loci (QTLs) specifying peak bone mass was performed with the F2 intercrosses of SAMP6, an established murine model of senile osteoporosis, exhibiting a significantly lower peak bone mass, and SAMP2, exhibiting a higher peak bone mass. Cortical thickness index (CTI), a parameter of bone mass of femurs, was measured in 488 F2 progeny at 4 months of age, when the animals attained peak bone mass by microphotodensitometry. Genetic markers were typed at 90 loci spanning all chromosomes except the Y. By interval mapping of 246 male F2 mice, two loci were identified with significant linkage to peak bone mass, one on Chromosome (Chr) 11 and another on Chr 13, with a maximum lod score of 10.8 (22.2% of the total variance) and 5.8 (10.0%), respectively. Another locus on the X Chr was suggestive of a QTL associated oppositely with a low peak bone mass to the SAMP2 allele. This association was consistent with the distribution of peak bone mass in the F1 and F2. These findings should be useful to elucidate the genetics of osteoporosis.

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalMammalian Genome
Volume10
Issue number2
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Quantitative Trait Loci
Bone and Bones
Osteoporosis
Lod Score
Chromosomes, Human, Pair 13
Chromosomes, Human, Pair 11
Y Chromosome
X Chromosome
Genetic Markers
Femur
Alleles
Genome

ASJC Scopus subject areas

  • Genetics

Cite this

Shimizu, M., Higuchi, K., Bennett, B., Xia, C., Tsuboyama, T., Kasai, S., ... Hosokawa, M. (1999). Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain. Mammalian Genome, 10(2), 81-87. https://doi.org/10.1007/s003359900949

Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain. / Shimizu, Motoyuki; Higuchi, Keiichi; Bennett, Beth; Xia, Chen; Tsuboyama, Tadao; Kasai, Soichiro; Chiba, Takuya; Fujisawa, Hiromi; Kogishi, Kumiko; Kitado, Haruo; Kimoto, Mitsutoshi; Takeda, Norikazu; Matsushita, Mutsumi; Okumura, Hideo; Serikawa, Tadao; Nakamura, Takashi; Johnson, Thomas E.; Hosokawa, Masanori.

In: Mammalian Genome, Vol. 10, No. 2, 1999, p. 81-87.

Research output: Contribution to journalArticle

Shimizu, M, Higuchi, K, Bennett, B, Xia, C, Tsuboyama, T, Kasai, S, Chiba, T, Fujisawa, H, Kogishi, K, Kitado, H, Kimoto, M, Takeda, N, Matsushita, M, Okumura, H, Serikawa, T, Nakamura, T, Johnson, TE & Hosokawa, M 1999, 'Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain', Mammalian Genome, vol. 10, no. 2, pp. 81-87. https://doi.org/10.1007/s003359900949
Shimizu M, Higuchi K, Bennett B, Xia C, Tsuboyama T, Kasai S et al. Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain. Mammalian Genome. 1999;10(2):81-87. https://doi.org/10.1007/s003359900949
Shimizu, Motoyuki ; Higuchi, Keiichi ; Bennett, Beth ; Xia, Chen ; Tsuboyama, Tadao ; Kasai, Soichiro ; Chiba, Takuya ; Fujisawa, Hiromi ; Kogishi, Kumiko ; Kitado, Haruo ; Kimoto, Mitsutoshi ; Takeda, Norikazu ; Matsushita, Mutsumi ; Okumura, Hideo ; Serikawa, Tadao ; Nakamura, Takashi ; Johnson, Thomas E. ; Hosokawa, Masanori. / Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain. In: Mammalian Genome. 1999 ; Vol. 10, No. 2. pp. 81-87.
@article{9d03207154b7488c89b972aa8223d3b2,
title = "Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain",
abstract = "The whole genome scan for quantitative trait loci (QTLs) specifying peak bone mass was performed with the F2 intercrosses of SAMP6, an established murine model of senile osteoporosis, exhibiting a significantly lower peak bone mass, and SAMP2, exhibiting a higher peak bone mass. Cortical thickness index (CTI), a parameter of bone mass of femurs, was measured in 488 F2 progeny at 4 months of age, when the animals attained peak bone mass by microphotodensitometry. Genetic markers were typed at 90 loci spanning all chromosomes except the Y. By interval mapping of 246 male F2 mice, two loci were identified with significant linkage to peak bone mass, one on Chromosome (Chr) 11 and another on Chr 13, with a maximum lod score of 10.8 (22.2{\%} of the total variance) and 5.8 (10.0{\%}), respectively. Another locus on the X Chr was suggestive of a QTL associated oppositely with a low peak bone mass to the SAMP2 allele. This association was consistent with the distribution of peak bone mass in the F1 and F2. These findings should be useful to elucidate the genetics of osteoporosis.",
author = "Motoyuki Shimizu and Keiichi Higuchi and Beth Bennett and Chen Xia and Tadao Tsuboyama and Soichiro Kasai and Takuya Chiba and Hiromi Fujisawa and Kumiko Kogishi and Haruo Kitado and Mitsutoshi Kimoto and Norikazu Takeda and Mutsumi Matsushita and Hideo Okumura and Tadao Serikawa and Takashi Nakamura and Johnson, {Thomas E.} and Masanori Hosokawa",
year = "1999",
doi = "10.1007/s003359900949",
language = "English",
volume = "10",
pages = "81--87",
journal = "Mammalian Genome",
issn = "0938-8990",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain

AU - Shimizu, Motoyuki

AU - Higuchi, Keiichi

AU - Bennett, Beth

AU - Xia, Chen

AU - Tsuboyama, Tadao

AU - Kasai, Soichiro

AU - Chiba, Takuya

AU - Fujisawa, Hiromi

AU - Kogishi, Kumiko

AU - Kitado, Haruo

AU - Kimoto, Mitsutoshi

AU - Takeda, Norikazu

AU - Matsushita, Mutsumi

AU - Okumura, Hideo

AU - Serikawa, Tadao

AU - Nakamura, Takashi

AU - Johnson, Thomas E.

AU - Hosokawa, Masanori

PY - 1999

Y1 - 1999

N2 - The whole genome scan for quantitative trait loci (QTLs) specifying peak bone mass was performed with the F2 intercrosses of SAMP6, an established murine model of senile osteoporosis, exhibiting a significantly lower peak bone mass, and SAMP2, exhibiting a higher peak bone mass. Cortical thickness index (CTI), a parameter of bone mass of femurs, was measured in 488 F2 progeny at 4 months of age, when the animals attained peak bone mass by microphotodensitometry. Genetic markers were typed at 90 loci spanning all chromosomes except the Y. By interval mapping of 246 male F2 mice, two loci were identified with significant linkage to peak bone mass, one on Chromosome (Chr) 11 and another on Chr 13, with a maximum lod score of 10.8 (22.2% of the total variance) and 5.8 (10.0%), respectively. Another locus on the X Chr was suggestive of a QTL associated oppositely with a low peak bone mass to the SAMP2 allele. This association was consistent with the distribution of peak bone mass in the F1 and F2. These findings should be useful to elucidate the genetics of osteoporosis.

AB - The whole genome scan for quantitative trait loci (QTLs) specifying peak bone mass was performed with the F2 intercrosses of SAMP6, an established murine model of senile osteoporosis, exhibiting a significantly lower peak bone mass, and SAMP2, exhibiting a higher peak bone mass. Cortical thickness index (CTI), a parameter of bone mass of femurs, was measured in 488 F2 progeny at 4 months of age, when the animals attained peak bone mass by microphotodensitometry. Genetic markers were typed at 90 loci spanning all chromosomes except the Y. By interval mapping of 246 male F2 mice, two loci were identified with significant linkage to peak bone mass, one on Chromosome (Chr) 11 and another on Chr 13, with a maximum lod score of 10.8 (22.2% of the total variance) and 5.8 (10.0%), respectively. Another locus on the X Chr was suggestive of a QTL associated oppositely with a low peak bone mass to the SAMP2 allele. This association was consistent with the distribution of peak bone mass in the F1 and F2. These findings should be useful to elucidate the genetics of osteoporosis.

UR - http://www.scopus.com/inward/record.url?scp=0011983514&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0011983514&partnerID=8YFLogxK

U2 - 10.1007/s003359900949

DO - 10.1007/s003359900949

M3 - Article

C2 - 9922384

AN - SCOPUS:0011983514

VL - 10

SP - 81

EP - 87

JO - Mammalian Genome

JF - Mammalian Genome

SN - 0938-8990

IS - 2

ER -