Identification of Rad51 alteration in patients with bilateral breast cancer

Masahiro Kato, Ken Ichi Yano, Fumie Matsuo, Hiroko Saito, Toyomasa Katagiri, Hitoshi Kurumizaka, Masataka Yoshimoto, Fujio Kasumi, Futoshi Akiyama, Goi Sakamoto, Hirokazu Nagawa, Yusuke Nakamura, Yoshio Miki

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The human Rad51 gene, HsRAD51, is a homolog of RecA of Escherichia coli and functions in recombination and DNA repair. BRCA1 and BRCA2 proteins form a complex with Rad51, and these genes are thought to participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Additionally, we have shown that the pattern of northern blot analysis of the Rad51 gene is closely similar to those of the BRCA1 and BRCA2 genes. It is therefore possible that alterations of the Rad51 gene may be involved in the development of hereditary breast cancer. To investigate this possibility, we screened Japanese patients with hereditary breast cancer for Rad51 mutations and found a single alteration in exon 6. This was determined to be present in the germline in two patients with bilateral breast cancer, one with synchronous bilateral breast cancer and the other with synchronous bilateral multiple breast cancer. In both patients, blood DNAs showed a G-to-A transition in the second nucleotide of codon 150, which results in the substitution of glutamine for arginine. As this alteration was not present in any patients with breast or colon cancer examined, we assume that this missense alteration is likely to be a disease-causing mutation.

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalJournal of Human Genetics
Volume45
Issue number3
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Breast Neoplasms
Genes
BRCA2 Protein
BRCA1 Protein
BRCA2 Gene
BRCA1 Gene
Recombinational DNA Repair
Mutation
Homologous Recombination
Glutamine
Codon
DNA Repair
Northern Blotting
Colonic Neoplasms
DNA Damage
Arginine
Exons
Nucleotides
Escherichia coli
DNA

Keywords

  • BRCA1
  • BRCA2
  • Germline alteration
  • Hereditary breast cancer
  • Rad51

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Kato, M., Yano, K. I., Matsuo, F., Saito, H., Katagiri, T., Kurumizaka, H., ... Miki, Y. (2000). Identification of Rad51 alteration in patients with bilateral breast cancer. Journal of Human Genetics, 45(3), 133-137.

Identification of Rad51 alteration in patients with bilateral breast cancer. / Kato, Masahiro; Yano, Ken Ichi; Matsuo, Fumie; Saito, Hiroko; Katagiri, Toyomasa; Kurumizaka, Hitoshi; Yoshimoto, Masataka; Kasumi, Fujio; Akiyama, Futoshi; Sakamoto, Goi; Nagawa, Hirokazu; Nakamura, Yusuke; Miki, Yoshio.

In: Journal of Human Genetics, Vol. 45, No. 3, 2000, p. 133-137.

Research output: Contribution to journalArticle

Kato, M, Yano, KI, Matsuo, F, Saito, H, Katagiri, T, Kurumizaka, H, Yoshimoto, M, Kasumi, F, Akiyama, F, Sakamoto, G, Nagawa, H, Nakamura, Y & Miki, Y 2000, 'Identification of Rad51 alteration in patients with bilateral breast cancer', Journal of Human Genetics, vol. 45, no. 3, pp. 133-137.
Kato M, Yano KI, Matsuo F, Saito H, Katagiri T, Kurumizaka H et al. Identification of Rad51 alteration in patients with bilateral breast cancer. Journal of Human Genetics. 2000;45(3):133-137.
Kato, Masahiro ; Yano, Ken Ichi ; Matsuo, Fumie ; Saito, Hiroko ; Katagiri, Toyomasa ; Kurumizaka, Hitoshi ; Yoshimoto, Masataka ; Kasumi, Fujio ; Akiyama, Futoshi ; Sakamoto, Goi ; Nagawa, Hirokazu ; Nakamura, Yusuke ; Miki, Yoshio. / Identification of Rad51 alteration in patients with bilateral breast cancer. In: Journal of Human Genetics. 2000 ; Vol. 45, No. 3. pp. 133-137.
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