Identification of region-specific transcription factor genes in the adult mouse brain by medium-scale real-time RT-PCR

Harukazu Suzuki, Rie Okunishi, Wataru Hashizume, Shintaro Katayama, Noriko Ninomiya, Naoki Osato, Kenjiro Sato, Mari Nakamura, Juri Iida, Mutsumi Kanamori, Yoshihide Hayashizaki

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

We established a medium-scale real-time RT-PCR system focusing on transcription factors and applied it to their expression profiles in the adult mouse 11 brain regions (http://genome.gsc.riken.jp/qRT-PCR/). Almost 90% of the examined genes showed significant expression in at least one region. We successfully extracted 179 region-specific genes by clustering analysis. Interestingly, the transcription factors involved in the development of the pituitary were still expressed in the adult pituitary, suggesting that they also play important roles in maintenance of the pituitary. These results provide unique molecular markers that may account for the molecular basis of the unique functions of specific brain regions.

Original languageEnglish
Pages (from-to)214-218
Number of pages5
JournalFEBS Letters
Volume573
Issue number1-3
DOIs
Publication statusPublished - 2004 Aug 27
Externally publishedYes

Keywords

  • Real-time RT-PCR
  • Region-specific
  • Transcription factor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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