Identification of symbiotically expressed coral mRNAs using a model infection system

Ikuko Yuyama, Hideki Hayakawa, Hirotoshi Endo, Kenji Iwao, Haruko Takeyama, Tadashi Maruyama, Toshiki Watanabe

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Hermatypic (or reef-building) corals live in obligatory mutualistic symbiosis with the symbiotic dinoflagellates Symbiodinium spp. (generally known as zooxanthellae). In an attempt to establish a model symbiosis system consisting of a coral host and a monoclonal population of zooxanthellae, infectivity of five cultured Symbiodinium cell lines was tested on naturally aposymbiotic juveniles of Acropora tenuis. A clade A3 strain (PL-TS-1) infected the juveniles at high density and promoted growth of the host. To identify host genes involved in the establishment or maintenance of symbiosis, mRNA expression patterns were compared between aposymbiotic and PL-TS-1-infected juvenile polyps using the suppression subtractive hybridization technique. Two mRNAs, the expression levels of which were augmented more than twofold by the presence of the symbionts, were thereby identified. One of the mRNAs, AtSym-02, encodes a novel protein of 322 amino acids which is predicted to be a glycosylated trans-membrane protein.

Original languageEnglish
Pages (from-to)793-798
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume336
Issue number3
DOIs
Publication statusPublished - 2005 Oct 28
Externally publishedYes

Fingerprint

Anthozoa
Symbiosis
Identification (control systems)
Messenger RNA
Infection
Coral Reefs
Dinoflagellida
Reefs
Polyps
Cultured Cells
Membrane Proteins
Genes
Cells
Maintenance
Amino Acids
Cell Line
Growth
Population
Proteins
titanium silicide

Keywords

  • cDNA cloning
  • Coral
  • Coral reef
  • Dinoflagellate
  • Gene expression
  • Symbiodinium
  • Symbiosis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of symbiotically expressed coral mRNAs using a model infection system. / Yuyama, Ikuko; Hayakawa, Hideki; Endo, Hirotoshi; Iwao, Kenji; Takeyama, Haruko; Maruyama, Tadashi; Watanabe, Toshiki.

In: Biochemical and Biophysical Research Communications, Vol. 336, No. 3, 28.10.2005, p. 793-798.

Research output: Contribution to journalArticle

Yuyama, Ikuko ; Hayakawa, Hideki ; Endo, Hirotoshi ; Iwao, Kenji ; Takeyama, Haruko ; Maruyama, Tadashi ; Watanabe, Toshiki. / Identification of symbiotically expressed coral mRNAs using a model infection system. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 336, No. 3. pp. 793-798.
@article{d7facdc5d58c42b0878ecec75546a02e,
title = "Identification of symbiotically expressed coral mRNAs using a model infection system",
abstract = "Hermatypic (or reef-building) corals live in obligatory mutualistic symbiosis with the symbiotic dinoflagellates Symbiodinium spp. (generally known as zooxanthellae). In an attempt to establish a model symbiosis system consisting of a coral host and a monoclonal population of zooxanthellae, infectivity of five cultured Symbiodinium cell lines was tested on naturally aposymbiotic juveniles of Acropora tenuis. A clade A3 strain (PL-TS-1) infected the juveniles at high density and promoted growth of the host. To identify host genes involved in the establishment or maintenance of symbiosis, mRNA expression patterns were compared between aposymbiotic and PL-TS-1-infected juvenile polyps using the suppression subtractive hybridization technique. Two mRNAs, the expression levels of which were augmented more than twofold by the presence of the symbionts, were thereby identified. One of the mRNAs, AtSym-02, encodes a novel protein of 322 amino acids which is predicted to be a glycosylated trans-membrane protein.",
keywords = "cDNA cloning, Coral, Coral reef, Dinoflagellate, Gene expression, Symbiodinium, Symbiosis",
author = "Ikuko Yuyama and Hideki Hayakawa and Hirotoshi Endo and Kenji Iwao and Haruko Takeyama and Tadashi Maruyama and Toshiki Watanabe",
year = "2005",
month = "10",
day = "28",
doi = "10.1016/j.bbrc.2005.08.174",
language = "English",
volume = "336",
pages = "793--798",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Identification of symbiotically expressed coral mRNAs using a model infection system

AU - Yuyama, Ikuko

AU - Hayakawa, Hideki

AU - Endo, Hirotoshi

AU - Iwao, Kenji

AU - Takeyama, Haruko

AU - Maruyama, Tadashi

AU - Watanabe, Toshiki

PY - 2005/10/28

Y1 - 2005/10/28

N2 - Hermatypic (or reef-building) corals live in obligatory mutualistic symbiosis with the symbiotic dinoflagellates Symbiodinium spp. (generally known as zooxanthellae). In an attempt to establish a model symbiosis system consisting of a coral host and a monoclonal population of zooxanthellae, infectivity of five cultured Symbiodinium cell lines was tested on naturally aposymbiotic juveniles of Acropora tenuis. A clade A3 strain (PL-TS-1) infected the juveniles at high density and promoted growth of the host. To identify host genes involved in the establishment or maintenance of symbiosis, mRNA expression patterns were compared between aposymbiotic and PL-TS-1-infected juvenile polyps using the suppression subtractive hybridization technique. Two mRNAs, the expression levels of which were augmented more than twofold by the presence of the symbionts, were thereby identified. One of the mRNAs, AtSym-02, encodes a novel protein of 322 amino acids which is predicted to be a glycosylated trans-membrane protein.

AB - Hermatypic (or reef-building) corals live in obligatory mutualistic symbiosis with the symbiotic dinoflagellates Symbiodinium spp. (generally known as zooxanthellae). In an attempt to establish a model symbiosis system consisting of a coral host and a monoclonal population of zooxanthellae, infectivity of five cultured Symbiodinium cell lines was tested on naturally aposymbiotic juveniles of Acropora tenuis. A clade A3 strain (PL-TS-1) infected the juveniles at high density and promoted growth of the host. To identify host genes involved in the establishment or maintenance of symbiosis, mRNA expression patterns were compared between aposymbiotic and PL-TS-1-infected juvenile polyps using the suppression subtractive hybridization technique. Two mRNAs, the expression levels of which were augmented more than twofold by the presence of the symbionts, were thereby identified. One of the mRNAs, AtSym-02, encodes a novel protein of 322 amino acids which is predicted to be a glycosylated trans-membrane protein.

KW - cDNA cloning

KW - Coral

KW - Coral reef

KW - Dinoflagellate

KW - Gene expression

KW - Symbiodinium

KW - Symbiosis

UR - http://www.scopus.com/inward/record.url?scp=25144443224&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25144443224&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2005.08.174

DO - 10.1016/j.bbrc.2005.08.174

M3 - Article

VL - 336

SP - 793

EP - 798

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -