Identification of the homozygously deleted region at chromosome 1p36.2 in human neuroblastoma

Akira Nakagawara, Miki Ohira, Hajime Kageyama, Motohiro Mihara, Shigeyuki Furuta, Taiichi Machida, Hajime Takayasu, Ashraful Islam, Yohko Nakamura, Masato Takahashi, Tomotane Shishikura, Yasuhiko Kaneko, Atsushi Toyoda, Masahira Hattori, Yoshiyuki Sakaki, Misao Ohki, Akira Horii, Eiichi Soeda, Johji Inazawa, Naohiko SekiHidekazu Kuma, Iwao Nozawa, Shigeru Sakiyama

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background. We have identified for the first time a homozygously deleted region within the smallest region of overlap at 1p36.2-3 in two neuroblastoma cell lines. Procedure. The 800kb PAC contig covering the entire homozygously deleted region was made and sequenced. To date, approximately 70% of sequencing has been accomplished, and the estimated length of the deleted region was 500 kb. Results. Currently, we have found six genes within the region, which include three known genes as well as three other genes that have been reported during processing of our present project for the last 3 1/2 years. We report here the results of expression and mutation analyses of those genes. Conclusions. Full sequencing for the region of homozygous deletion as well as further analyses of the genes mapped within the region may reveal whether or not there is a neuroblastoma suppressor gene as proposed by the Knudson's two-hit hypothesis. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)516-521
Number of pages6
JournalMedical and Pediatric Oncology
Volume35
Issue number6
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • 1p36
  • Homozygous deletion
  • Neuroblastoma
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Oncology
  • Cancer Research

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  • Cite this

    Nakagawara, A., Ohira, M., Kageyama, H., Mihara, M., Furuta, S., Machida, T., Takayasu, H., Islam, A., Nakamura, Y., Takahashi, M., Shishikura, T., Kaneko, Y., Toyoda, A., Hattori, M., Sakaki, Y., Ohki, M., Horii, A., Soeda, E., Inazawa, J., ... Sakiyama, S. (2000). Identification of the homozygously deleted region at chromosome 1p36.2 in human neuroblastoma. Medical and Pediatric Oncology, 35(6), 516-521. https://doi.org/10.1002/1096-911X(20001201)35:6<516::AID-MPO3>3.0.CO;2-Z