Identification of the regulator gene responsible for the acetone-responsive expression of the binuclear iron monooxygenase gene cluster in mycobacteria

Toshiki Furuya, Satomi Hirose, Hisashi Semba, Kuniki Kino

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    The mimABCD gene cluster encodes the binuclear iron monooxygenase that oxidizes propane and phenol in Mycobacterium smegmatis strain MC2 155 and Mycobacterium goodii strain 12523. Interestingly, expression of the mimABCD gene cluster is induced by acetone. In this study, we investigated the regulator gene responsible for this acetone-responsive expression. In the genome sequence of M. smegmatis strain MC2 155, the mimABCD gene cluster is preceded by a gene designated mimR, which is divergently transcribed. Sequence analysis revealed that MimR exhibits amino acid similarity with the NtrC family of transcriptional activators, including AcxR and AcoR, which are involved in acetone and acetoin metabolism, respectively. Unexpectedly, many homologs of the mimR gene were also found in the sequenced genomes of actinomycetes. A plasmid carrying a transcriptional fusion of the intergenic region between the mimR and mimA genes with a promoterless green fluorescent protein (GFP) gene was constructed and introduced into M. smegmatis strain MC2 155. Using a GFP reporter system, we confirmed by deletion and complementation analyses that the mimR gene product is the positive regulator of the mimABCD gene cluster expression that is responsive to acetone. M. goodii strain 12523 also utilized the same regulatory system as M. smegmatis strain MC2 155. Although transcriptional activators of the NtrC family generally control transcription using the σ 54 factor, a gene encoding the σ 54 factor was absent from the genome sequence of M. smegmatis strain MC2 155. These results suggest the presence of a novel regulatory system in actinomycetes, including mycobacteria.

    Original languageEnglish
    Pages (from-to)5817-5823
    Number of pages7
    JournalJournal of Bacteriology
    Volume193
    Issue number20
    DOIs
    Publication statusPublished - 2011 Oct

    Fingerprint

    Mycobacterium smegmatis
    Regulator Genes
    Mycobacterium
    Multigene Family
    Acetone
    Mixed Function Oxygenases
    Iron
    Genes
    Actinobacteria
    Genome
    Green Fluorescent Proteins
    Acetoin
    Propane
    Acinetobacter
    Intergenic DNA
    Phenol
    Sequence Analysis
    Plasmids
    Gene Expression
    Amino Acids

    ASJC Scopus subject areas

    • Microbiology
    • Molecular Biology

    Cite this

    Identification of the regulator gene responsible for the acetone-responsive expression of the binuclear iron monooxygenase gene cluster in mycobacteria. / Furuya, Toshiki; Hirose, Satomi; Semba, Hisashi; Kino, Kuniki.

    In: Journal of Bacteriology, Vol. 193, No. 20, 10.2011, p. 5817-5823.

    Research output: Contribution to journalArticle

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