Identification of the starting point for spermatogenesis resumption in the post-diapause development of the sweet potato hornworm, Agrius convolvuli L.

M. Kubo-Irie, T. Yamaguchi, Y. Tanaka, I. Yamazaki, M. Irie, H. Mohri, M. Shimoda

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    The resumption of spermatogenesis in post-diapause development was examined in the sweet potato hornworm (Agrius convolvuli) with in vivo bromodeoxyuridine (BrdU) incorporation experiments used to determine the starting point. Diapausing pupae were "overwintered" by chilling at 10. °C for over 4 months, after which they initiated post-diapause development by transferring the pupae to 25. °C with a 12-h light/12-h dark photoperiod. The testes of living, post-diapause pupae were injected with BrdU, which is incorporated into newly synthesized DNA strands. During the first 2 days after diapause termination, the nuclei of spermatogonia and spermatocytes failed to label with BrdU. However, on day 3 of post-diapause pupae (PDP3), labeling studies showed that cell proliferation was initiated by spermatogonia, but not by spermatocytes. In both hemolymph and testes, ecdysteroid concentrations rose gradually, reaching 0.3. μg/ml hemolymph at PDP3. These results led to the following three conclusions. The spermatogonial cell division is highly suppressed during diapause. After a long-term diapause, spermatogenesis resumes in the spermatogonia but not in the spermatocytes of diapause-terminated pupae. Cell division begins in advance of peak ecdysteroid concentrations. The latter result indicates that in post-diapause development, high concentrations of the hormone are not required to initiate spermatogonial proliferation.

    Original languageEnglish
    Pages (from-to)784-790
    Number of pages7
    JournalJournal of Insect Physiology
    Volume57
    Issue number6
    DOIs
    Publication statusPublished - 2011 Jun

    Fingerprint

    Convolvulus
    Agrius
    Ipomoea batatas
    Pupa
    Spermatogenesis
    sweet potatoes
    spermatogenesis
    diapause
    Spermatogonia
    Spermatocytes
    Bromodeoxyuridine
    Ecdysteroids
    pupae
    Hemolymph
    spermatogonia
    spermatocytes
    Cell Division
    Testis
    ecdysteroids
    Photoperiod

    Keywords

    • BrdU
    • Ecdysteroid
    • Hornworm
    • Post-diapause development
    • Spermatogonia

    ASJC Scopus subject areas

    • Insect Science
    • Physiology

    Cite this

    Identification of the starting point for spermatogenesis resumption in the post-diapause development of the sweet potato hornworm, Agrius convolvuli L. / Kubo-Irie, M.; Yamaguchi, T.; Tanaka, Y.; Yamazaki, I.; Irie, M.; Mohri, H.; Shimoda, M.

    In: Journal of Insect Physiology, Vol. 57, No. 6, 06.2011, p. 784-790.

    Research output: Contribution to journalArticle

    Kubo-Irie, M. ; Yamaguchi, T. ; Tanaka, Y. ; Yamazaki, I. ; Irie, M. ; Mohri, H. ; Shimoda, M. / Identification of the starting point for spermatogenesis resumption in the post-diapause development of the sweet potato hornworm, Agrius convolvuli L. In: Journal of Insect Physiology. 2011 ; Vol. 57, No. 6. pp. 784-790.
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