Identification of transposable elements contributing to tissue-specific expression of long non-coding RNAs

Takafumi Chishima, Junichi Iwakiri, Michiaki Hamada

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

It has been recently suggested that transposable elements (TEs) are re-used as functional elements of long non-coding RNAs (lncRNAs). This is supported by some examples such as the human endogenous retrovirus subfamily H (HERVH) elements contained within lncRNAs and expressed specifically in human embryonic stem cells (hESCs), as required to maintain hESC identity. There are at least two unanswered questions about all lncRNAs. How many TEs are re-used within lncRNAs? Are there any other TEs that affect tissue specificity of lncRNA expression? To answer these questions, we comprehensively identify TEs that are significantly related to tissue-specific expression levels of lncRNAs. We downloaded lncRNA expression data corresponding to normal human tissue from the Expression Atlas and transformed the data into tissue specificity estimates. Then, Fisher’s exact tests were performed to verify whether the presence or absence of TE-derived sequences influences the tissue specificity of lncRNA expression. Many TE–tissue pairs associated with tissue-specific expression of lncRNAs were detected, indicating that multiple TE families can be re-used as functional domains or regulatory sequences of lncRNAs. In particular, we found that the antisense promoter region of L1PA2, a LINE-1 subfamily, appears to act as a promoter for lncRNAs with placenta-specific expression.

Original languageEnglish
Article number23
JournalGenes
Volume9
Issue number1
DOIs
Publication statusPublished - 2018 Jan

Keywords

  • Long non-coding RNA
  • Tissue-specific expression
  • Transposable element

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'Identification of transposable elements contributing to tissue-specific expression of long non-coding RNAs'. Together they form a unique fingerprint.

Cite this