Identification of two novel members of erbA superfamily by molecular cloning: The gene products of the two are highly related to each other

Nobuyuki Miyajtma, Yasunori Kandowaki, Shin Ichi Fukushige, Sho Ichi Shimizu, Kentaro Senba, Yuji Yamanashi, Ken Ichi Matsubara, Kumao Toyoshima, Tadashi Yamamoto

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Two v-erbA-related genes, named ear-2 and ear-3, have been identified in the human genome and characterized by cDNA cloning. These genes are predicted to encode proteins that are very similar in primary structure to receptors for steroid hormones or thyroid hormone (T3) In addition, amino acid sequences of the ear-2 and ear-3 gene products are very similar each other especially at the DNA binding domain (86% homology) and at the putative ligand binding domain (76% homology). Northern hybridization with ear DNA probes of RNAs from various tissues of a human fetus reveals that the expression of ear-2 is high in the liver whereas the expression of ear-3 is relatively ubiquitous. Hybridization analysis of DNAs from sorted chromosomes shows that the ear-2 gene is located on chromosome 19 and ear-3 on chromosome 5, indicating that the two genes are clearly different from each other.

Original languageEnglish
Pages (from-to)11057-11074
Number of pages18
JournalNucleic Acids Research
Volume16
Issue number23
DOIs
Publication statusPublished - 1988 Dec 9
Externally publishedYes

Fingerprint

Cloning
Molecular Cloning
Ear
Genes
Gene
Chromosome
Chromosomes
Hormones
DNA
Homology
CDNA Cloning
Steroid hormones
Steroids
Amino Acid Sequence
erbA Genes
Receptor
DNA Probes
Liver
Triiodothyronine
RNA

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Identification of two novel members of erbA superfamily by molecular cloning : The gene products of the two are highly related to each other. / Miyajtma, Nobuyuki; Kandowaki, Yasunori; Fukushige, Shin Ichi; Shimizu, Sho Ichi; Senba, Kentaro; Yamanashi, Yuji; Matsubara, Ken Ichi; Toyoshima, Kumao; Yamamoto, Tadashi.

In: Nucleic Acids Research, Vol. 16, No. 23, 09.12.1988, p. 11057-11074.

Research output: Contribution to journalArticle

Miyajtma, N, Kandowaki, Y, Fukushige, SI, Shimizu, SI, Senba, K, Yamanashi, Y, Matsubara, KI, Toyoshima, K & Yamamoto, T 1988, 'Identification of two novel members of erbA superfamily by molecular cloning: The gene products of the two are highly related to each other', Nucleic Acids Research, vol. 16, no. 23, pp. 11057-11074. https://doi.org/10.1093/nar/16.23.11057
Miyajtma, Nobuyuki ; Kandowaki, Yasunori ; Fukushige, Shin Ichi ; Shimizu, Sho Ichi ; Senba, Kentaro ; Yamanashi, Yuji ; Matsubara, Ken Ichi ; Toyoshima, Kumao ; Yamamoto, Tadashi. / Identification of two novel members of erbA superfamily by molecular cloning : The gene products of the two are highly related to each other. In: Nucleic Acids Research. 1988 ; Vol. 16, No. 23. pp. 11057-11074.
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