Identification of two novel members of erbA superfamily by molecular cloning: The gene products of the two are highly related to each other

Nobuyuki Miyajtma, Yasunori Kandowaki, Shin Ichi Fukushige, Sho Ichi Shimizu, Kentaro Semba, Yuji Yamanashi, Ken Ichi Matsubara, Kumao Toyoshima, Tadashi Yamamoto

Research output: Contribution to journalArticlepeer-review

165 Citations (Scopus)

Abstract

Two v-erbA-related genes, named ear-2 and ear-3, have been identified in the human genome and characterized by cDNA cloning. These genes are predicted to encode proteins that are very similar in primary structure to receptors for steroid hormones or thyroid hormone (T3) In addition, amino acid sequences of the ear-2 and ear-3 gene products are very similar each other especially at the DNA binding domain (86% homology) and at the putative ligand binding domain (76% homology). Northern hybridization with ear DNA probes of RNAs from various tissues of a human fetus reveals that the expression of ear-2 is high in the liver whereas the expression of ear-3 is relatively ubiquitous. Hybridization analysis of DNAs from sorted chromosomes shows that the ear-2 gene is located on chromosome 19 and ear-3 on chromosome 5, indicating that the two genes are clearly different from each other.

Original languageEnglish
Pages (from-to)11057-11074
Number of pages18
JournalNucleic acids research
Volume16
Issue number23
DOIs
Publication statusPublished - 1988 Dec 9
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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