Abstract
We recently helped to complete the sequence of human chromosome 21 at a very high level of accuracy. Using this sequence we identified two novel genes, designated DSCR9 and DSCR10, in the so-called Down Syndrome Critical Region (DSCR) by computational gene prediction and subsequent cDNA cloning. Both DSCR9 and DSCR10 are expressed preferentially in testis and encode functionally unknown proteins with 149 and 87 amino acid residues, respectively. Zoo blot analysis suggested that both genes are exclusive to primate genomes such as chimpanzee, gorilla, orangutan, crab-eating monkey and African green monkey but are not present in other non-primate mammals including mouse, dog, cat, and chicken. Comparative genomic sequence analysis of DSCR9 and DSCR10 with the corresponding mouse syntenic region confirmed the lack of these genes in the mouse. These results strongly suggest that DSCR9 and DSCR10 have emerged as a new class of gene in the primate lineage during evolution.
| Original language | English |
|---|---|
| Pages (from-to) | 89-97 |
| Number of pages | 9 |
| Journal | DNA Research |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2002 |
| Externally published | Yes |
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Keywords
- CDNA cloning
- Down Syndrome Critical Region
- Primate-specific genes
ASJC Scopus subject areas
- Genetics
- Molecular Biology
Cite this
Identification of two novel primate-specific genes in DSCR. / Takamatsu, Kunihiko; Maekawa, Kohei; Togashi, Takushi; Choi, Dong Kug; Suzuki, Yutaka; Taylor, Todd D.; Toyoda, Atsushi; Sugano, Sumio; Fujiyama, Asao; Hattori, Masahira; Sakaki, Yoshiyuki; Takeda, Tadayuki.
In: DNA Research, Vol. 9, No. 3, 2002, p. 89-97.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of two novel primate-specific genes in DSCR
AU - Takamatsu, Kunihiko
AU - Maekawa, Kohei
AU - Togashi, Takushi
AU - Choi, Dong Kug
AU - Suzuki, Yutaka
AU - Taylor, Todd D.
AU - Toyoda, Atsushi
AU - Sugano, Sumio
AU - Fujiyama, Asao
AU - Hattori, Masahira
AU - Sakaki, Yoshiyuki
AU - Takeda, Tadayuki
PY - 2002
Y1 - 2002
N2 - We recently helped to complete the sequence of human chromosome 21 at a very high level of accuracy. Using this sequence we identified two novel genes, designated DSCR9 and DSCR10, in the so-called Down Syndrome Critical Region (DSCR) by computational gene prediction and subsequent cDNA cloning. Both DSCR9 and DSCR10 are expressed preferentially in testis and encode functionally unknown proteins with 149 and 87 amino acid residues, respectively. Zoo blot analysis suggested that both genes are exclusive to primate genomes such as chimpanzee, gorilla, orangutan, crab-eating monkey and African green monkey but are not present in other non-primate mammals including mouse, dog, cat, and chicken. Comparative genomic sequence analysis of DSCR9 and DSCR10 with the corresponding mouse syntenic region confirmed the lack of these genes in the mouse. These results strongly suggest that DSCR9 and DSCR10 have emerged as a new class of gene in the primate lineage during evolution.
AB - We recently helped to complete the sequence of human chromosome 21 at a very high level of accuracy. Using this sequence we identified two novel genes, designated DSCR9 and DSCR10, in the so-called Down Syndrome Critical Region (DSCR) by computational gene prediction and subsequent cDNA cloning. Both DSCR9 and DSCR10 are expressed preferentially in testis and encode functionally unknown proteins with 149 and 87 amino acid residues, respectively. Zoo blot analysis suggested that both genes are exclusive to primate genomes such as chimpanzee, gorilla, orangutan, crab-eating monkey and African green monkey but are not present in other non-primate mammals including mouse, dog, cat, and chicken. Comparative genomic sequence analysis of DSCR9 and DSCR10 with the corresponding mouse syntenic region confirmed the lack of these genes in the mouse. These results strongly suggest that DSCR9 and DSCR10 have emerged as a new class of gene in the primate lineage during evolution.
KW - CDNA cloning
KW - Down Syndrome Critical Region
KW - Primate-specific genes
UR - http://www.scopus.com/inward/record.url?scp=0036932523&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036932523&partnerID=8YFLogxK
U2 - 10.1093/dnares/9.3.89
DO - 10.1093/dnares/9.3.89
M3 - Article
C2 - 12168953
AN - SCOPUS:0036932523
VL - 9
SP - 89
EP - 97
JO - DNA Research
JF - DNA Research
SN - 1340-2838
IS - 3
ER -