Identification of zinc finger transcription factor EGR2 as a novel acetylated protein

Kota Noritsugu, Akihiro Ito, Youichi Nakao, Minoru Yoshida

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    EGR2 is a zinc finger transcription factor that regulates myelination in the peripheral nervous system and T cell anergy. The transcriptional activity of EGR2 is known to be regulated by its co-activators and/or co-repressors. Although the activity of transcription factors is generally regulated not only by interactions with co-regulators but also posttranslational modifications including acetylation, little is known about posttranslational modifications of EGR2. Here we show that EGR2 is a novel acetylated protein. Through immunoblotting analyses using an antibody that specifically recognizes the acetylated form of EGR2, CBP and p300 were identified as acetyltransferases, while HDAC6, 10 and SIRT1 were identified as deacetylases of EGR2. Although the NuRD complex containing HDAC1 and HDAC2 is known to associate with EGR2, the present study suggests that acetylation of EGR2 is regulated independently of NuRD.

    Original languageEnglish
    Pages (from-to)455-459
    Number of pages5
    JournalBiochemical and Biophysical Research Communications
    Volume489
    Issue number4
    DOIs
    Publication statusPublished - 2017 Aug 5

    Keywords

    • Acetylation
    • EGR2
    • HAT
    • HDAC

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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