ik3-1/cables is a substrate for cyclin-dependent kinase 3 (cdk 3)

Tadanori Yamochi, Kentaro Senba, Keitaro Tsuji, Kiyohisa Mizumoto, Hiroko Sato, Yoshiharu Matsuura, Ikuo Nishimoto, Masaaki Matsuoka

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

p70ik3-1 (a 70-kDa protein) contains a cyclin box, and binds to p35cdk3 in vivo and in vitro [Matsuoka, M., Matsuura, Y., Semba, K. & Nishimoto, I. (2000) Biochem. Biophys. Res. Commun. 273, 442-447]. In spite of its structural similarity to cyclins, p70ik3-1 does not activate cyclin-dependent kinase 3 (cdk3)-mediated phosphorylation of pRb, histone H1, or the C-terminal domain of RNA polymerase II. Here, we report that Ser274 of p70ik3-1 is phosphorylated by cdk2 or cdk3 bound to cyclin A and to cyclin E in vitro. We also found that in COS7 cells in which cyclin E and cdk3 were ectopically overexpressed, the phosphorylation level of Ser274 in coexpressed p70ik3-1 is upregulated. We therefore conclude that p70ik3-1 is a substrate for cdk3-mediated phosphorylation.

Original languageEnglish
Pages (from-to)6076-6082
Number of pages7
JournalEuropean Journal of Biochemistry
Volume268
Issue number23
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Cyclin-Dependent Kinase 3
Phosphorylation
Cables
Cyclin E
Cyclins
Substrates
Cyclin A
RNA Polymerase II
Histones
Proteins

Keywords

  • cdk3
  • ik3-1
  • Phosphorylation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yamochi, T., Senba, K., Tsuji, K., Mizumoto, K., Sato, H., Matsuura, Y., ... Matsuoka, M. (2001). ik3-1/cables is a substrate for cyclin-dependent kinase 3 (cdk 3). European Journal of Biochemistry, 268(23), 6076-6082. https://doi.org/10.1046/j.0014-2956.2001.02555.x

ik3-1/cables is a substrate for cyclin-dependent kinase 3 (cdk 3). / Yamochi, Tadanori; Senba, Kentaro; Tsuji, Keitaro; Mizumoto, Kiyohisa; Sato, Hiroko; Matsuura, Yoshiharu; Nishimoto, Ikuo; Matsuoka, Masaaki.

In: European Journal of Biochemistry, Vol. 268, No. 23, 2001, p. 6076-6082.

Research output: Contribution to journalArticle

Yamochi, T, Senba, K, Tsuji, K, Mizumoto, K, Sato, H, Matsuura, Y, Nishimoto, I & Matsuoka, M 2001, 'ik3-1/cables is a substrate for cyclin-dependent kinase 3 (cdk 3)', European Journal of Biochemistry, vol. 268, no. 23, pp. 6076-6082. https://doi.org/10.1046/j.0014-2956.2001.02555.x
Yamochi, Tadanori ; Senba, Kentaro ; Tsuji, Keitaro ; Mizumoto, Kiyohisa ; Sato, Hiroko ; Matsuura, Yoshiharu ; Nishimoto, Ikuo ; Matsuoka, Masaaki. / ik3-1/cables is a substrate for cyclin-dependent kinase 3 (cdk 3). In: European Journal of Biochemistry. 2001 ; Vol. 268, No. 23. pp. 6076-6082.
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AU - Senba, Kentaro

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AU - Sato, Hiroko

AU - Matsuura, Yoshiharu

AU - Nishimoto, Ikuo

AU - Matsuoka, Masaaki

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AB - p70ik3-1 (a 70-kDa protein) contains a cyclin box, and binds to p35cdk3 in vivo and in vitro [Matsuoka, M., Matsuura, Y., Semba, K. & Nishimoto, I. (2000) Biochem. Biophys. Res. Commun. 273, 442-447]. In spite of its structural similarity to cyclins, p70ik3-1 does not activate cyclin-dependent kinase 3 (cdk3)-mediated phosphorylation of pRb, histone H1, or the C-terminal domain of RNA polymerase II. Here, we report that Ser274 of p70ik3-1 is phosphorylated by cdk2 or cdk3 bound to cyclin A and to cyclin E in vitro. We also found that in COS7 cells in which cyclin E and cdk3 were ectopically overexpressed, the phosphorylation level of Ser274 in coexpressed p70ik3-1 is upregulated. We therefore conclude that p70ik3-1 is a substrate for cdk3-mediated phosphorylation.

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