IL-12 involvement in myogenic differentiation of C2C12 in vitro

Sara Romanazzo, Giancarlo Forte, Keisuke Morishima, Akiyoshi Taniguchi

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Recently, the extracellular microenvironment has been shown to be critical for the correct differentiation of stem cells to specific tissues. Many factors, including physical (e.g. biomaterial stiffness and topography) and biological (as growth factors, cytokines and chemokines) components, cooperate to create an ideal microenvironment for muscle stem cells, with many of these factors having been widely investigated. We previously demonstrated that the use of non-proliferating muscle-specific and unrelated cells as feeder layers for skeletal muscle progenitor cell differentiation resulted in significant differences in the ability to form myotubes, suggesting the importance of biological factors in myogenic differentiation. In this study, we investigated the biological factors involved in this process, analyzing the expression profile of 84 genes coding for cytokines and chemokines. We successfully identified a novel role for the cytokine IL-12 in the myogenic differentiation of C2C12 mouse skeletal muscle cells. Experiments involving the overexpression or silencing of the IL-12 gene in C2C12 showed that IL-12 enhanced the myogenic differentiation process. Moreover, when IL-12 was overexpressed in non-biologically related feeder cells, the new co-culture system was able to improve myogenic differentiation of C2C12 seeded on top. Although IL-12 is known to be a cytokine involved in inflammatory responses, it also appears to be involved in the myogenic differentiation process, acting as a positive regulator of this mechanism. This fact is expected to prove to be important for the development of functional biomaterials. This journal is

    Original languageEnglish
    Pages (from-to)469-479
    Number of pages11
    JournalBiomaterials Science
    Volume3
    Issue number3
    DOIs
    Publication statusPublished - 2015 Mar 1

    Fingerprint

    Interleukin-12
    Muscle
    Cytokines
    Feeder Cells
    Stem cells
    Biomaterials
    Biological Factors
    Biocompatible Materials
    Chemokines
    Genes
    Topography
    Cells
    Stiffness
    Tissue
    Intercellular Signaling Peptides and Proteins
    Experiments

    ASJC Scopus subject areas

    • Materials Science(all)
    • Biomedical Engineering

    Cite this

    IL-12 involvement in myogenic differentiation of C2C12 in vitro. / Romanazzo, Sara; Forte, Giancarlo; Morishima, Keisuke; Taniguchi, Akiyoshi.

    In: Biomaterials Science, Vol. 3, No. 3, 01.03.2015, p. 469-479.

    Research output: Contribution to journalArticle

    Romanazzo, Sara ; Forte, Giancarlo ; Morishima, Keisuke ; Taniguchi, Akiyoshi. / IL-12 involvement in myogenic differentiation of C2C12 in vitro. In: Biomaterials Science. 2015 ; Vol. 3, No. 3. pp. 469-479.
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