IL-17, neutrophil activation and muscle damage following endurance exercise

Kaoru Sugama, Katsuhiko Suzuki, Kayo Yoshitani, Koso Shiraishi, Takashi Kometani

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The T-cell subset Th17 is induced partly by interleukin (IL)-6 and activated by IL- 23, and produces a proinflammatory cytokine IL-17. Since IL-6 increases dramatically following long-lasting endurance exercise, this response may also stimulate the induction of IL-17 and IL-23 after exercise. The aim of this study was to clarify the dynamics of IL-17 in association with endurance exercise-induced muscle damage and inflammatory responses. Fourteen male triathletes participated in a duathlon race consisting of 5 km of running, 40 km of cycling and 5 km of running. Venous blood and urine samples were collected before, immediately after, 1.5 h and 3 h after the race. Plasma and urine were analyzed using enzyme-linked immunosorbent assays (ELISA). Haematological and biochemical variables such as neutrophil activation marker (myeloperoxidase: MPO), muscle damage marker (myoglobin: Mb) and soluble receptor activator of nuclear factor (NF)-κB ligand (sRANKL) were also determined to estimate the biological and pathological significance. Plasma concentrations of IL-6 (+26.0×), MPO (+3.2×) and Mb (+4.9×) increased significantly immediately after the race and IL-17 and IL-23 tended to increase. Furthermore, plasma concentrations of IL-12p40 and sRANKL increased significantly after the race. The measured parameters related to Th17 cytokines in the urinary output were closely correlated with each other and muscle damage marker. These findings suggest that IL-17 induced by IL-6 and activated by IL-23 or other IL-17 producing-cells and IL-23 might promote neutrophil activation and muscle damage following prolonged endurance exercise.

Original languageEnglish
Pages (from-to)115-126
Number of pages12
JournalExercise Immunology Review
Volume18
Publication statusPublished - 2012

Fingerprint

Neutrophil Activation
Interleukin-17
Interleukin-23
Exercise
Muscles
Interleukin-6
Running
Urine
Cytokines
Myoglobin
Interleukins
T-Lymphocyte Subsets
Cytoplasmic and Nuclear Receptors
Peroxidase
Enzyme-Linked Immunosorbent Assay
Ligands

Keywords

  • Helper T (Th) cells cytokine balance
  • Interleukin (IL)-17
  • Muscle damage
  • Neutrophil activation
  • Urinary excretion

ASJC Scopus subject areas

  • Immunology

Cite this

Sugama, K., Suzuki, K., Yoshitani, K., Shiraishi, K., & Kometani, T. (2012). IL-17, neutrophil activation and muscle damage following endurance exercise. Exercise Immunology Review, 18, 115-126.

IL-17, neutrophil activation and muscle damage following endurance exercise. / Sugama, Kaoru; Suzuki, Katsuhiko; Yoshitani, Kayo; Shiraishi, Koso; Kometani, Takashi.

In: Exercise Immunology Review, Vol. 18, 2012, p. 115-126.

Research output: Contribution to journalArticle

Sugama, K, Suzuki, K, Yoshitani, K, Shiraishi, K & Kometani, T 2012, 'IL-17, neutrophil activation and muscle damage following endurance exercise', Exercise Immunology Review, vol. 18, pp. 115-126.
Sugama, Kaoru ; Suzuki, Katsuhiko ; Yoshitani, Kayo ; Shiraishi, Koso ; Kometani, Takashi. / IL-17, neutrophil activation and muscle damage following endurance exercise. In: Exercise Immunology Review. 2012 ; Vol. 18. pp. 115-126.
@article{2be6165b8b3b4ae192ec0c18cda3473f,
title = "IL-17, neutrophil activation and muscle damage following endurance exercise",
abstract = "The T-cell subset Th17 is induced partly by interleukin (IL)-6 and activated by IL- 23, and produces a proinflammatory cytokine IL-17. Since IL-6 increases dramatically following long-lasting endurance exercise, this response may also stimulate the induction of IL-17 and IL-23 after exercise. The aim of this study was to clarify the dynamics of IL-17 in association with endurance exercise-induced muscle damage and inflammatory responses. Fourteen male triathletes participated in a duathlon race consisting of 5 km of running, 40 km of cycling and 5 km of running. Venous blood and urine samples were collected before, immediately after, 1.5 h and 3 h after the race. Plasma and urine were analyzed using enzyme-linked immunosorbent assays (ELISA). Haematological and biochemical variables such as neutrophil activation marker (myeloperoxidase: MPO), muscle damage marker (myoglobin: Mb) and soluble receptor activator of nuclear factor (NF)-κB ligand (sRANKL) were also determined to estimate the biological and pathological significance. Plasma concentrations of IL-6 (+26.0×), MPO (+3.2×) and Mb (+4.9×) increased significantly immediately after the race and IL-17 and IL-23 tended to increase. Furthermore, plasma concentrations of IL-12p40 and sRANKL increased significantly after the race. The measured parameters related to Th17 cytokines in the urinary output were closely correlated with each other and muscle damage marker. These findings suggest that IL-17 induced by IL-6 and activated by IL-23 or other IL-17 producing-cells and IL-23 might promote neutrophil activation and muscle damage following prolonged endurance exercise.",
keywords = "Helper T (Th) cells cytokine balance, Interleukin (IL)-17, Muscle damage, Neutrophil activation, Urinary excretion",
author = "Kaoru Sugama and Katsuhiko Suzuki and Kayo Yoshitani and Koso Shiraishi and Takashi Kometani",
year = "2012",
language = "English",
volume = "18",
pages = "115--126",
journal = "Exercise Immunology Review",
issn = "1077-5552",
publisher = "Human Kinetics Publishers Inc.",

}

TY - JOUR

T1 - IL-17, neutrophil activation and muscle damage following endurance exercise

AU - Sugama, Kaoru

AU - Suzuki, Katsuhiko

AU - Yoshitani, Kayo

AU - Shiraishi, Koso

AU - Kometani, Takashi

PY - 2012

Y1 - 2012

N2 - The T-cell subset Th17 is induced partly by interleukin (IL)-6 and activated by IL- 23, and produces a proinflammatory cytokine IL-17. Since IL-6 increases dramatically following long-lasting endurance exercise, this response may also stimulate the induction of IL-17 and IL-23 after exercise. The aim of this study was to clarify the dynamics of IL-17 in association with endurance exercise-induced muscle damage and inflammatory responses. Fourteen male triathletes participated in a duathlon race consisting of 5 km of running, 40 km of cycling and 5 km of running. Venous blood and urine samples were collected before, immediately after, 1.5 h and 3 h after the race. Plasma and urine were analyzed using enzyme-linked immunosorbent assays (ELISA). Haematological and biochemical variables such as neutrophil activation marker (myeloperoxidase: MPO), muscle damage marker (myoglobin: Mb) and soluble receptor activator of nuclear factor (NF)-κB ligand (sRANKL) were also determined to estimate the biological and pathological significance. Plasma concentrations of IL-6 (+26.0×), MPO (+3.2×) and Mb (+4.9×) increased significantly immediately after the race and IL-17 and IL-23 tended to increase. Furthermore, plasma concentrations of IL-12p40 and sRANKL increased significantly after the race. The measured parameters related to Th17 cytokines in the urinary output were closely correlated with each other and muscle damage marker. These findings suggest that IL-17 induced by IL-6 and activated by IL-23 or other IL-17 producing-cells and IL-23 might promote neutrophil activation and muscle damage following prolonged endurance exercise.

AB - The T-cell subset Th17 is induced partly by interleukin (IL)-6 and activated by IL- 23, and produces a proinflammatory cytokine IL-17. Since IL-6 increases dramatically following long-lasting endurance exercise, this response may also stimulate the induction of IL-17 and IL-23 after exercise. The aim of this study was to clarify the dynamics of IL-17 in association with endurance exercise-induced muscle damage and inflammatory responses. Fourteen male triathletes participated in a duathlon race consisting of 5 km of running, 40 km of cycling and 5 km of running. Venous blood and urine samples were collected before, immediately after, 1.5 h and 3 h after the race. Plasma and urine were analyzed using enzyme-linked immunosorbent assays (ELISA). Haematological and biochemical variables such as neutrophil activation marker (myeloperoxidase: MPO), muscle damage marker (myoglobin: Mb) and soluble receptor activator of nuclear factor (NF)-κB ligand (sRANKL) were also determined to estimate the biological and pathological significance. Plasma concentrations of IL-6 (+26.0×), MPO (+3.2×) and Mb (+4.9×) increased significantly immediately after the race and IL-17 and IL-23 tended to increase. Furthermore, plasma concentrations of IL-12p40 and sRANKL increased significantly after the race. The measured parameters related to Th17 cytokines in the urinary output were closely correlated with each other and muscle damage marker. These findings suggest that IL-17 induced by IL-6 and activated by IL-23 or other IL-17 producing-cells and IL-23 might promote neutrophil activation and muscle damage following prolonged endurance exercise.

KW - Helper T (Th) cells cytokine balance

KW - Interleukin (IL)-17

KW - Muscle damage

KW - Neutrophil activation

KW - Urinary excretion

UR - http://www.scopus.com/inward/record.url?scp=84862087759&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862087759&partnerID=8YFLogxK

M3 - Article

C2 - 22876724

AN - SCOPUS:84862087759

VL - 18

SP - 115

EP - 126

JO - Exercise Immunology Review

JF - Exercise Immunology Review

SN - 1077-5552

ER -