Imaging mGluR5 dynamics in astrocytes using quantum dots

Misa Arizono, Hiroko Bannai, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This unit describes the method that we have developed to clarify endogenous mGluR5 (metabotropic glutamate receptors 5) dynamics in astrocytes by single-particle tracking using quantum dots (QD-SPT). QD-SPT has been a powerful tool to examine the contribution of neurotransmitter receptor dynamics to synaptic plasticity. Neurotransmitter receptors are also expressed in astrocytes, the most abundant form of glial cell in the brain. mGluR5s, which evoke intracellular Ca2+ signals upon receiving glutamate, contribute to the modulation of synaptic transmission efficacy and local blood flow by astrocytes. QD-SPT has previously revealed that the regulation of the lateral diffusion of mGluR5 on the plasma membrane is important for local Ca2+ signaling in astrocytes. Determining how mGluR5 dynamics are regulated in response to neuronal input would enable a better understanding of neuron-astrocyte communication in future studies.

Original languageEnglish
Article number2.21
JournalCurrent Protocols in Neuroscience
Issue numberSUPPL.66
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Astrocyte
  • mGluR5
  • Quantum dot
  • Single particle tracking

ASJC Scopus subject areas

  • Neuroscience(all)

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