Imaging of Mitotic Spindle Dynamics in Caenorhabditis elegans Embryos

Mika Toya, Yumi Iida, Asako Sugimoto

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Development of the nematode Caenorhabditis elegans is highly reproducible, and the cell division patterns are virtually invariant. Transparency of the eggshell and cells enables the observation of intracellular events with a high temporal and spatial resolution. These unique features, along with the sophisticated genetic techniques, make this organism one of the most attractive model systems for dissecting regulatory mechanisms of dynamic cellular behaviors, such as mitosis, at an organismal level. In this chapter, we describe immunofluorescence and live imaging methods for analyzing mitotic spindle regulation. In particular, we present the use of double- or triple-labeled fluorescent strains for high-resolution two-dimensional and three-dimensional live imaging to analyze dynamic behaviors of mitotic spindles.

Original languageEnglish
Pages (from-to)359-372
Number of pages14
JournalMethods in Cell Biology
Volume97
Issue numberC
DOIs
Publication statusPublished - 2010 Aug 20

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ASJC Scopus subject areas

  • Cell Biology

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