Imaging the lateral diffusion of membrane molecules with quantum dots

Hiroko Bannai, Sabine Lévi, Claude Schweizer, Maxime Dahan, Antoine Triller

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

This protocol describes a sensitive approach to tracking the motion of membrane molecules such as lipids and proteins with molecular resolution in live cells. This technique makes use of fluorescent semiconductor nanocrystals, quantum dots (QDs), as a probe to detect membrane molecules of interest. The photostability and brightness of QDs allow them to be tracked at a single particle level for longer periods than previous fluorophores, such as fluorescent proteins and organic dyes. QDs are bound to the extracellular part of the object to be followed, and their movements can be recorded with a fluorescence microscope equipped with a spectral lamp and a sensitive cooled charge-coupled device camera. The experimental procedure described for neurons below takes about 45 min. This technique is applicable to various cultured cells.

Original languageEnglish
Pages (from-to)2628-2634
Number of pages7
JournalNature protocols
Volume1
Issue number6
DOIs
Publication statusPublished - 2007 Jan
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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