Immobilization of target-bound aptamer on field effect transistor biosensor to improve sensitivity for detection of uncharged cortisol

Hiroki Hayashi, Ryo Toyama, Ryota Takibuchi, Sho Hideshima, Shigeki Kuroiwa, Naoto Kaneko, Katsunori Horii, Keishi Ohashi, Toshiyuki Momma, Tetsuya Osaka

Research output: Contribution to journalArticlepeer-review

Abstract

Field effect transistor (FET) biosensors are capable of detecting various biomolecules, although challenges remain in the detection of uncharged molecules. In this study, the detection of uncharged cortisol was demonstrated by interfacial design using a technique to immobilize target-bound aptamers. The target-bound aptamers, which formed a higher-order structure than target-unbound aptamers, expanded the distance between adjacent aptamers and reduced the steric hindrance to the conformational change. The density-controlled aptamers efficiently induced their conformational changes with the cortisol binding, which resulted in the improvement of the sensitivity of FET biosensors.

Original languageEnglish
Pages (from-to)134-137
Number of pages4
JournalElectrochemistry
Volume89
Issue number2
DOIs
Publication statusPublished - 2021 Mar 5

Keywords

  • Aptamer
  • Conformational change
  • Cortisol
  • Field effect transistor

ASJC Scopus subject areas

  • Electrochemistry

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